Human intraovarian interleukin-1 (IL-1) system: Highly compartmentalized and hormonally dependent regulation of the genes encoding IL-1, its receptor, and its receptor antagonist

Arye Hurwitz, Jill Loukides, Elisabetta Ricciarelli, Luis Botero, Eugene Katz, Jan M. McAllister, Jairo E. Garcia, Richard Rohan, Eli Y. Adashi, Eleuterio R. Hernandez

Research output: Contribution to journalArticle

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Abstract

To delineate the scope of the human intraovarian IL-1 system we used a solution hybridization/RNase protection assay to test for expression of the genes encoding IL-1, its type I receptor (IL-1R), and its receptor antagonist (IL-1RA). IL-1 transcripts were not detected in whole ovarian material from days 4 or 12 of an unstimulated menstrual cycle but transcripts (IL-1β ≫ IL-11α) were detected in preovulatory follicular aspirates from gonadotropin-stimulated cycles. Concurrently obtained peripheral monocytes did not contain IL-1β transcripts but macrophage-depleted follicular aspirates did, thus implicating the granulosa cells as the site of IL-1 expression. IL-1R transcripts were detected in RNA from whole ovaries and follicular aspirates but not in RNA from peripheral monocytes. IL-1 RA transcripts were detected in whole ovarian material as well as in macrophage-free follicular aspirates. Cultured human granulosa and theca cells did not contain mRNA for IL-1β or IL-1RA but did contain mRNA for IL-1R. Treatment of cell cultures with forskolin (25 μM) induced IL-1β transcripts in granulosa but not theca cells. Forskolin also increased the basal levels of IL-1R transcripts in both granulosa and theca cells but did not induce IL-RA transcripts in either cell type. Taken together, these findings reveal the existence of a complete, highly compartmentalized, hormonally dependent intraovarian IL-1 system replete with ligands, receptor, and receptor antagonist.

Original languageEnglish (US)
Pages (from-to)1746-1754
Number of pages9
JournalJournal of Clinical Investigation
Volume89
Issue number6
DOIs
StatePublished - Jan 1 1992

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Interleukin-1 Receptors
Interleukin-1
Genes
Theca Cells
Granulosa Cells
Colforsin
Monocytes
Interleukin-1 Type I Receptors
Macrophages
RNA
Interleukin-11
Messenger RNA
Ribonucleases
Menstrual Cycle
Gonadotropins
Ovary
Cell Culture Techniques
Ligands
Gene Expression

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Hurwitz, Arye ; Loukides, Jill ; Ricciarelli, Elisabetta ; Botero, Luis ; Katz, Eugene ; McAllister, Jan M. ; Garcia, Jairo E. ; Rohan, Richard ; Adashi, Eli Y. ; Hernandez, Eleuterio R. / Human intraovarian interleukin-1 (IL-1) system : Highly compartmentalized and hormonally dependent regulation of the genes encoding IL-1, its receptor, and its receptor antagonist. In: Journal of Clinical Investigation. 1992 ; Vol. 89, No. 6. pp. 1746-1754.
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abstract = "To delineate the scope of the human intraovarian IL-1 system we used a solution hybridization/RNase protection assay to test for expression of the genes encoding IL-1, its type I receptor (IL-1R), and its receptor antagonist (IL-1RA). IL-1 transcripts were not detected in whole ovarian material from days 4 or 12 of an unstimulated menstrual cycle but transcripts (IL-1β ≫ IL-11α) were detected in preovulatory follicular aspirates from gonadotropin-stimulated cycles. Concurrently obtained peripheral monocytes did not contain IL-1β transcripts but macrophage-depleted follicular aspirates did, thus implicating the granulosa cells as the site of IL-1 expression. IL-1R transcripts were detected in RNA from whole ovaries and follicular aspirates but not in RNA from peripheral monocytes. IL-1 RA transcripts were detected in whole ovarian material as well as in macrophage-free follicular aspirates. Cultured human granulosa and theca cells did not contain mRNA for IL-1β or IL-1RA but did contain mRNA for IL-1R. Treatment of cell cultures with forskolin (25 μM) induced IL-1β transcripts in granulosa but not theca cells. Forskolin also increased the basal levels of IL-1R transcripts in both granulosa and theca cells but did not induce IL-RA transcripts in either cell type. Taken together, these findings reveal the existence of a complete, highly compartmentalized, hormonally dependent intraovarian IL-1 system replete with ligands, receptor, and receptor antagonist.",
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Human intraovarian interleukin-1 (IL-1) system : Highly compartmentalized and hormonally dependent regulation of the genes encoding IL-1, its receptor, and its receptor antagonist. / Hurwitz, Arye; Loukides, Jill; Ricciarelli, Elisabetta; Botero, Luis; Katz, Eugene; McAllister, Jan M.; Garcia, Jairo E.; Rohan, Richard; Adashi, Eli Y.; Hernandez, Eleuterio R.

In: Journal of Clinical Investigation, Vol. 89, No. 6, 01.01.1992, p. 1746-1754.

Research output: Contribution to journalArticle

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T2 - Highly compartmentalized and hormonally dependent regulation of the genes encoding IL-1, its receptor, and its receptor antagonist

AU - Hurwitz, Arye

AU - Loukides, Jill

AU - Ricciarelli, Elisabetta

AU - Botero, Luis

AU - Katz, Eugene

AU - McAllister, Jan M.

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AU - Rohan, Richard

AU - Adashi, Eli Y.

AU - Hernandez, Eleuterio R.

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