Human papillomavirus in amniotic fluid

Mack Ruffin, Joanne M. Bailey, Diane Roulston, Daisy R. Lee, Ruth Ann Tucker, David C. Swan, Elizabeth R. Unger

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: There is evidence to suggest that human papillomavirus (HPV) can cross the placenta resulting in in-utero transmission. The goal of this study was to determine if HPV can be detected in amniotic fluid from women with intact amniotic membranes. Methods: Residual amniotic fluid and cultured cell pellets from amniocentesis performed for prenatal diagnosis were used. PGMY09/11 L1 consensus primers and GP5+/GP6+ primers were used in a nested polymerase chain reaction assay for HPV. Results: There were 146 paired samples from 142 women representing 139 singleton pregnancies, 2 twin pregnancies, and 1 triplet pregnancy. The women were 78% Caucasian, 5% African American, 14% Asian, and 2% Hispanic. The average age was 35.2 years with a range of 23-55 years. All samples were β-globin positive. HPV was not detected in any of the paired samples. Conclusion: Given the age range, race, and ethnicity of the study population, one would anticipate some evidence of HPV if it could easily cross the placenta, but there was none.

Original languageEnglish (US)
Article number28
JournalBMC Pregnancy and Childbirth
Volume6
DOIs
StatePublished - Sep 4 2006

Fingerprint

Amniotic Fluid
Placenta
Triplet Pregnancy
Twin Pregnancy
Amnion
Amniocentesis
Globins
Prenatal Diagnosis
Hispanic Americans
African Americans
Cultured Cells
Pregnancy
Polymerase Chain Reaction
Population

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Ruffin, M., Bailey, J. M., Roulston, D., Lee, D. R., Tucker, R. A., Swan, D. C., & Unger, E. R. (2006). Human papillomavirus in amniotic fluid. BMC Pregnancy and Childbirth, 6, [28]. https://doi.org/10.1186/1471-2393-6-28
Ruffin, Mack ; Bailey, Joanne M. ; Roulston, Diane ; Lee, Daisy R. ; Tucker, Ruth Ann ; Swan, David C. ; Unger, Elizabeth R. / Human papillomavirus in amniotic fluid. In: BMC Pregnancy and Childbirth. 2006 ; Vol. 6.
@article{cb83a1d822b64787a9ad9afcc82feda0,
title = "Human papillomavirus in amniotic fluid",
abstract = "Background: There is evidence to suggest that human papillomavirus (HPV) can cross the placenta resulting in in-utero transmission. The goal of this study was to determine if HPV can be detected in amniotic fluid from women with intact amniotic membranes. Methods: Residual amniotic fluid and cultured cell pellets from amniocentesis performed for prenatal diagnosis were used. PGMY09/11 L1 consensus primers and GP5+/GP6+ primers were used in a nested polymerase chain reaction assay for HPV. Results: There were 146 paired samples from 142 women representing 139 singleton pregnancies, 2 twin pregnancies, and 1 triplet pregnancy. The women were 78{\%} Caucasian, 5{\%} African American, 14{\%} Asian, and 2{\%} Hispanic. The average age was 35.2 years with a range of 23-55 years. All samples were β-globin positive. HPV was not detected in any of the paired samples. Conclusion: Given the age range, race, and ethnicity of the study population, one would anticipate some evidence of HPV if it could easily cross the placenta, but there was none.",
author = "Mack Ruffin and Bailey, {Joanne M.} and Diane Roulston and Lee, {Daisy R.} and Tucker, {Ruth Ann} and Swan, {David C.} and Unger, {Elizabeth R.}",
year = "2006",
month = "9",
day = "4",
doi = "10.1186/1471-2393-6-28",
language = "English (US)",
volume = "6",
journal = "BMC Pregnancy and Childbirth",
issn = "1471-2393",
publisher = "BioMed Central",

}

Ruffin, M, Bailey, JM, Roulston, D, Lee, DR, Tucker, RA, Swan, DC & Unger, ER 2006, 'Human papillomavirus in amniotic fluid', BMC Pregnancy and Childbirth, vol. 6, 28. https://doi.org/10.1186/1471-2393-6-28

Human papillomavirus in amniotic fluid. / Ruffin, Mack; Bailey, Joanne M.; Roulston, Diane; Lee, Daisy R.; Tucker, Ruth Ann; Swan, David C.; Unger, Elizabeth R.

In: BMC Pregnancy and Childbirth, Vol. 6, 28, 04.09.2006.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human papillomavirus in amniotic fluid

AU - Ruffin, Mack

AU - Bailey, Joanne M.

AU - Roulston, Diane

AU - Lee, Daisy R.

AU - Tucker, Ruth Ann

AU - Swan, David C.

AU - Unger, Elizabeth R.

PY - 2006/9/4

Y1 - 2006/9/4

N2 - Background: There is evidence to suggest that human papillomavirus (HPV) can cross the placenta resulting in in-utero transmission. The goal of this study was to determine if HPV can be detected in amniotic fluid from women with intact amniotic membranes. Methods: Residual amniotic fluid and cultured cell pellets from amniocentesis performed for prenatal diagnosis were used. PGMY09/11 L1 consensus primers and GP5+/GP6+ primers were used in a nested polymerase chain reaction assay for HPV. Results: There were 146 paired samples from 142 women representing 139 singleton pregnancies, 2 twin pregnancies, and 1 triplet pregnancy. The women were 78% Caucasian, 5% African American, 14% Asian, and 2% Hispanic. The average age was 35.2 years with a range of 23-55 years. All samples were β-globin positive. HPV was not detected in any of the paired samples. Conclusion: Given the age range, race, and ethnicity of the study population, one would anticipate some evidence of HPV if it could easily cross the placenta, but there was none.

AB - Background: There is evidence to suggest that human papillomavirus (HPV) can cross the placenta resulting in in-utero transmission. The goal of this study was to determine if HPV can be detected in amniotic fluid from women with intact amniotic membranes. Methods: Residual amniotic fluid and cultured cell pellets from amniocentesis performed for prenatal diagnosis were used. PGMY09/11 L1 consensus primers and GP5+/GP6+ primers were used in a nested polymerase chain reaction assay for HPV. Results: There were 146 paired samples from 142 women representing 139 singleton pregnancies, 2 twin pregnancies, and 1 triplet pregnancy. The women were 78% Caucasian, 5% African American, 14% Asian, and 2% Hispanic. The average age was 35.2 years with a range of 23-55 years. All samples were β-globin positive. HPV was not detected in any of the paired samples. Conclusion: Given the age range, race, and ethnicity of the study population, one would anticipate some evidence of HPV if it could easily cross the placenta, but there was none.

UR - http://www.scopus.com/inward/record.url?scp=33748853666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748853666&partnerID=8YFLogxK

U2 - 10.1186/1471-2393-6-28

DO - 10.1186/1471-2393-6-28

M3 - Article

C2 - 16952308

AN - SCOPUS:33748853666

VL - 6

JO - BMC Pregnancy and Childbirth

JF - BMC Pregnancy and Childbirth

SN - 1471-2393

M1 - 28

ER -

Ruffin M, Bailey JM, Roulston D, Lee DR, Tucker RA, Swan DC et al. Human papillomavirus in amniotic fluid. BMC Pregnancy and Childbirth. 2006 Sep 4;6. 28. https://doi.org/10.1186/1471-2393-6-28