Human papillomavirus type 45 propagation, infection, and neutralization

Margaret E. McLaughlin-Drubin, Susan Wilson, Brian Mullikin, Jo Ann Suzich, Craig Meyers

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The organotypic (raft) culture system has allowed the study of the entire differentiation-dependent life cycle of human papillomaviruses (HPVs), including virion morphogenesis. We introduced linearized HPV45 genomic DNA into primary keratinocytes, where it recircularized and maintained episomally at a range of 10-50 copies of HPV genomic DNA. Following epithelial stratification and differentiation in organotypic culture, virion morphogenesis occurred. HPV45 virions were purified from raft cultures and were able to infect keratinocytes in vitro. By testing a panel of HPV VLP antisera, we were able to demonstrate that the infection was neutralized not only with human HPV45 VLP-specific antiserum, but also with human HPV18 VLP-specific antiserum, demonstrating serological cross-reactivity between HPV18 and HPV45.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalVirology
Volume312
Issue number1
DOIs
StatePublished - Jul 20 2003

All Science Journal Classification (ASJC) codes

  • Virology

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