Human papillomavirus type 6 virus-like particles present overlapping yet distinct conformational epitopes

Xin Min Wang, James C. Cook, Jessica C. Lee, Kathrin U. Jansen, Neil D. Christensen, Steven W. Ludmerer, William L. Clements

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The epitope for a human papillomavirus (HPV) type 6 conformation-dependent, neutralizing monoclonal antibody (mAb) was partially mapped using HPV L1 recombinant virus-like particles (VLPs). The mAb H6.J54 is cross-reactive with the closely related HPV types 6 and 11. By making HPV-6-like amino acid substitutions in the cottontail rabbit papillomavirus (CRPV) major capsid protein L1, we were able to transfer H6.J54 binding activity into a CRPV/HPV-6 hybrid L1 protein. Full binding activity was achieved with only nine amino acid changes and identified a region centred on the HPV-6 residues 49-54. This region has previously been shown to be a critical part of HPV-6 type-specific epitopes. Fine mapping of the region by scanning a series of alanine substitution mutations showed that in HPV-6 VLPs this type-common epitope overlaps HPV-6 type-specific epitopes.

Original languageEnglish (US)
Pages (from-to)1493-1497
Number of pages5
JournalJournal of General Virology
Volume84
Issue number6
DOIs
StatePublished - Jun 1 2003

All Science Journal Classification (ASJC) codes

  • Virology

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