Human serum albumin adsorption study on 62-MHz miniaturized quartz gravimetric sensors

Ping Kao, Ashish Patwardhan, David Allara, Srinivas Tadigadapa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have designed and fabricated 25-μm-thick quartz resonators operating at a fundamental resonance frequency of ∼62 MHz. The results show a substantial increase in the mass sensitivity compared to single monolithic commercial resonators operating at lower frequencies in the ∼5-10-MHz range. The overall performance of the micromachined resonators is demonstrated for the example of human serum albumin protein adsorption from aqueous buffer solutions onto gold electrodes functionalized with self-assembled monolayers. The results show a saturation adsorption frequency change of 6.8 kHz as opposed to 40 Hz for a commercial ∼5-MHz sensor under identical loading conditions. From the analysis of the adsorption isotherm, the equilibrium adsorption constant of the adsorption of the protein layer was found to be K = 8.03 × 10 ∧6 M∧-1, which is in agreement with the values reported in the literature. The high sensitivity of the miniaturized QCM devices can be a significant advantage in both vapor and solution adsorption analyses.

Original languageEnglish (US)
Pages (from-to)5930-5936
Number of pages7
JournalAnalytical chemistry
Volume80
Issue number15
DOIs
StatePublished - Aug 1 2008

Fingerprint

Quartz
Serum Albumin
Adsorption
Sensors
Resonators
Self assembled monolayers
Adsorption isotherms
Gold
Buffers
Proteins
Vapors
Electrodes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Kao, Ping ; Patwardhan, Ashish ; Allara, David ; Tadigadapa, Srinivas. / Human serum albumin adsorption study on 62-MHz miniaturized quartz gravimetric sensors. In: Analytical chemistry. 2008 ; Vol. 80, No. 15. pp. 5930-5936.
@article{04fbccbde54243899b3edee0ef0672a4,
title = "Human serum albumin adsorption study on 62-MHz miniaturized quartz gravimetric sensors",
abstract = "We have designed and fabricated 25-μm-thick quartz resonators operating at a fundamental resonance frequency of ∼62 MHz. The results show a substantial increase in the mass sensitivity compared to single monolithic commercial resonators operating at lower frequencies in the ∼5-10-MHz range. The overall performance of the micromachined resonators is demonstrated for the example of human serum albumin protein adsorption from aqueous buffer solutions onto gold electrodes functionalized with self-assembled monolayers. The results show a saturation adsorption frequency change of 6.8 kHz as opposed to 40 Hz for a commercial ∼5-MHz sensor under identical loading conditions. From the analysis of the adsorption isotherm, the equilibrium adsorption constant of the adsorption of the protein layer was found to be K = 8.03 × 10 ∧6 M∧-1, which is in agreement with the values reported in the literature. The high sensitivity of the miniaturized QCM devices can be a significant advantage in both vapor and solution adsorption analyses.",
author = "Ping Kao and Ashish Patwardhan and David Allara and Srinivas Tadigadapa",
year = "2008",
month = "8",
day = "1",
doi = "10.1021/ac8005395",
language = "English (US)",
volume = "80",
pages = "5930--5936",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "15",

}

Kao, P, Patwardhan, A, Allara, D & Tadigadapa, S 2008, 'Human serum albumin adsorption study on 62-MHz miniaturized quartz gravimetric sensors', Analytical chemistry, vol. 80, no. 15, pp. 5930-5936. https://doi.org/10.1021/ac8005395

Human serum albumin adsorption study on 62-MHz miniaturized quartz gravimetric sensors. / Kao, Ping; Patwardhan, Ashish; Allara, David; Tadigadapa, Srinivas.

In: Analytical chemistry, Vol. 80, No. 15, 01.08.2008, p. 5930-5936.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human serum albumin adsorption study on 62-MHz miniaturized quartz gravimetric sensors

AU - Kao, Ping

AU - Patwardhan, Ashish

AU - Allara, David

AU - Tadigadapa, Srinivas

PY - 2008/8/1

Y1 - 2008/8/1

N2 - We have designed and fabricated 25-μm-thick quartz resonators operating at a fundamental resonance frequency of ∼62 MHz. The results show a substantial increase in the mass sensitivity compared to single monolithic commercial resonators operating at lower frequencies in the ∼5-10-MHz range. The overall performance of the micromachined resonators is demonstrated for the example of human serum albumin protein adsorption from aqueous buffer solutions onto gold electrodes functionalized with self-assembled monolayers. The results show a saturation adsorption frequency change of 6.8 kHz as opposed to 40 Hz for a commercial ∼5-MHz sensor under identical loading conditions. From the analysis of the adsorption isotherm, the equilibrium adsorption constant of the adsorption of the protein layer was found to be K = 8.03 × 10 ∧6 M∧-1, which is in agreement with the values reported in the literature. The high sensitivity of the miniaturized QCM devices can be a significant advantage in both vapor and solution adsorption analyses.

AB - We have designed and fabricated 25-μm-thick quartz resonators operating at a fundamental resonance frequency of ∼62 MHz. The results show a substantial increase in the mass sensitivity compared to single monolithic commercial resonators operating at lower frequencies in the ∼5-10-MHz range. The overall performance of the micromachined resonators is demonstrated for the example of human serum albumin protein adsorption from aqueous buffer solutions onto gold electrodes functionalized with self-assembled monolayers. The results show a saturation adsorption frequency change of 6.8 kHz as opposed to 40 Hz for a commercial ∼5-MHz sensor under identical loading conditions. From the analysis of the adsorption isotherm, the equilibrium adsorption constant of the adsorption of the protein layer was found to be K = 8.03 × 10 ∧6 M∧-1, which is in agreement with the values reported in the literature. The high sensitivity of the miniaturized QCM devices can be a significant advantage in both vapor and solution adsorption analyses.

UR - http://www.scopus.com/inward/record.url?scp=49449083001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49449083001&partnerID=8YFLogxK

U2 - 10.1021/ac8005395

DO - 10.1021/ac8005395

M3 - Article

C2 - 18570386

AN - SCOPUS:49449083001

VL - 80

SP - 5930

EP - 5936

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 15

ER -