Human SP-A

Genotype and single-strand conformation polymorphism analysis

L. Krizkova, R. Sakthivel, S. A. Olowe, P. K. Rogan, Joanna Floros

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have previously characterized two surfactant protein A (SP-A) cDNAs termed 1A and 6A, as well as a 6A allelic variant termed 6A 1 . These sequences are quite heterogeneous at the 3' untranslated region (3'UT). Differences between 6A and 6A 1 alleles include an 11-bp insertion/deletion 407 bases downstream from the start of the translation termination codon and a base pair polymorphism (C or G) in exon 1 (position 1,193; White, Damm, Miller, Spratt, Schilling, Hawgood, Benson, and Cordell. Nature Lond. 317: 361-363, 1985). The 11-bp (GCCCACTGCCT) segment is present in 6A 1 and absent in 6A. The 6A/6A genotype, in a small number of specimens, showed a trend toward a higher frequency in the black Nigerian population compared with Caucasians. In this report, we examine the frequency of the 6A genotype in a larger number of samples from Caucasians and black Nigerians as well as the meiotic stability of the 3'UT heterogeneity. Slot-blot analysis and allele- specific oligonucleotide probes have confirmed that the 6A/6A genotype is more frequent in the Nigerian population. Single-strand conformation polymorphisms in the 3'UT appear to be stably inherited by members of a three-generation family, suggesting that these nucleotide variants represent natural polymorphisms in the population.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume266
Issue number5 10-5
StatePublished - Jan 1 1994

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Pulmonary Surfactant-Associated Protein A
3' Untranslated Regions
Genotype
Alleles
Population
Oligonucleotide Probes
Terminator Codon
Base Pairing
Exons
Nucleotides
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

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title = "Human SP-A: Genotype and single-strand conformation polymorphism analysis",
abstract = "We have previously characterized two surfactant protein A (SP-A) cDNAs termed 1A and 6A, as well as a 6A allelic variant termed 6A 1 . These sequences are quite heterogeneous at the 3' untranslated region (3'UT). Differences between 6A and 6A 1 alleles include an 11-bp insertion/deletion 407 bases downstream from the start of the translation termination codon and a base pair polymorphism (C or G) in exon 1 (position 1,193; White, Damm, Miller, Spratt, Schilling, Hawgood, Benson, and Cordell. Nature Lond. 317: 361-363, 1985). The 11-bp (GCCCACTGCCT) segment is present in 6A 1 and absent in 6A. The 6A/6A genotype, in a small number of specimens, showed a trend toward a higher frequency in the black Nigerian population compared with Caucasians. In this report, we examine the frequency of the 6A genotype in a larger number of samples from Caucasians and black Nigerians as well as the meiotic stability of the 3'UT heterogeneity. Slot-blot analysis and allele- specific oligonucleotide probes have confirmed that the 6A/6A genotype is more frequent in the Nigerian population. Single-strand conformation polymorphisms in the 3'UT appear to be stably inherited by members of a three-generation family, suggesting that these nucleotide variants represent natural polymorphisms in the population.",
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Human SP-A : Genotype and single-strand conformation polymorphism analysis. / Krizkova, L.; Sakthivel, R.; Olowe, S. A.; Rogan, P. K.; Floros, Joanna.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 266, No. 5 10-5, 01.01.1994.

Research output: Contribution to journalArticle

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AU - Floros, Joanna

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