Human telomerase reverse transcriptase (hTERT) transcription requires Sp1/Sp3 binding to the promoter and a permissive chromatin environment

De Cheng, Yuanjun Zhao, Shuwen Wang, Wenwen Jia, Jiuhong Kang, Jiyue Zhu

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Thetranscription ofhumantelomerase genehTERTis regulated by transcription factors (TFs), including Sp1 family proteins, and its chromatin environment. To understand its regulation in a relevant chromatin context, we employed bacterial artificial chromosome reporters containing 160 kb of human genomic sequence containing the hTERT gene. Upon chromosomal integration, the bacterial artificial chromosomes recapitulated endogenous hTERT expression, contrary to transient reporters. Sp1/Sp3 expression did not correlate with hTERT promoter activity, and these TFs bound to the hTERT promoters in both telomerase-positive and telomerase-negative cells. Mutation of the proximal GCbox resulted in a dramatic decrease of hTERT promoter activity, and mutations of all five GC-boxes eliminated its transcriptional activity. Neither mutations of GC-boxes nor knockdown of endogenous Sp1 impacted promoter binding by other TFs, including E-box-binding proteins, and histone acetylation and trimethylation of histone H3K9 at the hTERT promoter in telomerase-positive and -negative cells. The result indicated that promoter binding by Sp1/Sp3 was essential, but not a limiting step, for hTERT transcription. hTERT transcription required a permissive chromatin environment. Importantly, our data also revealed different functions of GC-boxes and E-boxes inhTERTregulation; althoughGCboxes were essential for promoter activity, factors bound to the E-boxes functioned to de-repress hTERT promoter.

Original languageEnglish (US)
Pages (from-to)30193-30203
Number of pages11
JournalJournal of Biological Chemistry
Volume290
Issue number50
DOIs
StatePublished - Dec 11 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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