Humoral immune response to Plasmodium falciparum merozoite surface protein 1 C-terminal region based hybrid nucleic acid vaccines in mice

Jun Miao, Xun Li, Caifang Xue

Research output: Contribution to journalArticle

Abstract

Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/ HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition. Methods BALB/c or C57BL/6 mice received three intramuscular immunizations with 200μg/100μl or 100μg/100μl of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. HG or MSP1-17 antibodies were monitored by indirect ELISA. The effect of immune sera on parasite inhibition was monitored by in vitro inhibition assays. Results BALB/c and C57BL/6 mice immunized with 100μg/100μl of VR1012/HG-MSP1-17 per mouse gave significantly HG and MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200μg/100μl of VR1012/HG-MSP1-17 per mouse gave higher HG antibodies but no change in MSP1-17 antibodies. BALB/c immunized with 200μg/100μl of VR1012/TPA/HG-MSP1-17 per mouse gave low level of HG antibodies and no MSP1-17 antibodies. Sera from the mice immunized with 200μg/100μl VR1012/HG-MSP1-17 could significantly inhibit Plasmodium falciparum growth. Conclusion VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17. The sera of VR1012/HG-MSP1-17 immunized mice could inhibit parasite growth prominently.

Original languageEnglish (US)
Pages (from-to)156-159
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume20
Issue number2
StatePublished - Mar 2000

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Merozoite Surface Protein 1
DNA Vaccines
Plasmodium falciparum
Humoral Immunity
Protein C
Antibodies
Parasites
Inbred C57BL Mouse
Immune Sera

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

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title = "Humoral immune response to Plasmodium falciparum merozoite surface protein 1 C-terminal region based hybrid nucleic acid vaccines in mice",
abstract = "Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/ HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition. Methods BALB/c or C57BL/6 mice received three intramuscular immunizations with 200μg/100μl or 100μg/100μl of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. HG or MSP1-17 antibodies were monitored by indirect ELISA. The effect of immune sera on parasite inhibition was monitored by in vitro inhibition assays. Results BALB/c and C57BL/6 mice immunized with 100μg/100μl of VR1012/HG-MSP1-17 per mouse gave significantly HG and MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200μg/100μl of VR1012/HG-MSP1-17 per mouse gave higher HG antibodies but no change in MSP1-17 antibodies. BALB/c immunized with 200μg/100μl of VR1012/TPA/HG-MSP1-17 per mouse gave low level of HG antibodies and no MSP1-17 antibodies. Sera from the mice immunized with 200μg/100μl VR1012/HG-MSP1-17 could significantly inhibit Plasmodium falciparum growth. Conclusion VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17. The sera of VR1012/HG-MSP1-17 immunized mice could inhibit parasite growth prominently.",
author = "Jun Miao and Xun Li and Caifang Xue",
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journal = "Chinese Journal of Microbiology and Immunology",
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Humoral immune response to Plasmodium falciparum merozoite surface protein 1 C-terminal region based hybrid nucleic acid vaccines in mice. / Miao, Jun; Li, Xun; Xue, Caifang.

In: Chinese Journal of Microbiology and Immunology, Vol. 20, No. 2, 03.2000, p. 156-159.

Research output: Contribution to journalArticle

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T1 - Humoral immune response to Plasmodium falciparum merozoite surface protein 1 C-terminal region based hybrid nucleic acid vaccines in mice

AU - Miao, Jun

AU - Li, Xun

AU - Xue, Caifang

PY - 2000/3

Y1 - 2000/3

N2 - Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/ HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition. Methods BALB/c or C57BL/6 mice received three intramuscular immunizations with 200μg/100μl or 100μg/100μl of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. HG or MSP1-17 antibodies were monitored by indirect ELISA. The effect of immune sera on parasite inhibition was monitored by in vitro inhibition assays. Results BALB/c and C57BL/6 mice immunized with 100μg/100μl of VR1012/HG-MSP1-17 per mouse gave significantly HG and MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200μg/100μl of VR1012/HG-MSP1-17 per mouse gave higher HG antibodies but no change in MSP1-17 antibodies. BALB/c immunized with 200μg/100μl of VR1012/TPA/HG-MSP1-17 per mouse gave low level of HG antibodies and no MSP1-17 antibodies. Sera from the mice immunized with 200μg/100μl VR1012/HG-MSP1-17 could significantly inhibit Plasmodium falciparum growth. Conclusion VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17. The sera of VR1012/HG-MSP1-17 immunized mice could inhibit parasite growth prominently.

AB - Objective To analyze humoral immune response to nucleic acid vaccines (VR1012/TPA/ HG-MSP1-17 for intracellular expression or VR1012/HG-MSP1-17 for secretion) which contain Plasmodium falciparum Merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL-1 and TT and to monitor the effect of DNA-immune sera on parasite inhibition. Methods BALB/c or C57BL/6 mice received three intramuscular immunizations with 200μg/100μl or 100μg/100μl of VR1012/HG-MSP1-17 or VR1012/TPA/HG-MSP1-17 per mouse each time. HG or MSP1-17 antibodies were monitored by indirect ELISA. The effect of immune sera on parasite inhibition was monitored by in vitro inhibition assays. Results BALB/c and C57BL/6 mice immunized with 100μg/100μl of VR1012/HG-MSP1-17 per mouse gave significantly HG and MSP1-17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200μg/100μl of VR1012/HG-MSP1-17 per mouse gave higher HG antibodies but no change in MSP1-17 antibodies. BALB/c immunized with 200μg/100μl of VR1012/TPA/HG-MSP1-17 per mouse gave low level of HG antibodies and no MSP1-17 antibodies. Sera from the mice immunized with 200μg/100μl VR1012/HG-MSP1-17 could significantly inhibit Plasmodium falciparum growth. Conclusion VR1012/HG-MSP1-17 is more immunogenic than VR1012/TPA/HG-MSP1-17. The sera of VR1012/HG-MSP1-17 immunized mice could inhibit parasite growth prominently.

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