Hybridization-based karyotyping of mouse chromosomes: Hybridization-bands

Melissa C. Liechty, C. M. Carpio, S. Aytay, A. C. Clase, K. L. Puschus, K. R. Sims, L. M. Davis, J. C. Hozier

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have developed a method, which we have named hybridization-banding, to identify simultaneously all chromosomes in a mouse metaphase spread. The method uses a combination of hybridization probes labeled with a single fluor to yield a simple, unique, readily identifiable hybridization pattern on each chromosome. The method is superior to Giemsa- or fluorescence-based banding methods for chromosome identification because the hybridization patterns are simpler and easier to identify, and unique patterns can be designed at will for each chromosome. Analysis can be performed with a standard fluorescence microscope, and images can be recorded on film with an ordinary 35-mm camera, making the method useful to many investigators. The method can also be applied to any species for which chromosomes and probes can be prepared.

Original languageEnglish (US)
Pages (from-to)34-38
Number of pages5
JournalCytogenetics and Cell Genetics
Volume86
Issue number1
StatePublished - Sep 14 1999

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Karyotyping
Chromosomes
Fluorescence
Chromosome Banding
Metaphase
Research Personnel

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Liechty, M. C., Carpio, C. M., Aytay, S., Clase, A. C., Puschus, K. L., Sims, K. R., ... Hozier, J. C. (1999). Hybridization-based karyotyping of mouse chromosomes: Hybridization-bands. Cytogenetics and Cell Genetics, 86(1), 34-38.
Liechty, Melissa C. ; Carpio, C. M. ; Aytay, S. ; Clase, A. C. ; Puschus, K. L. ; Sims, K. R. ; Davis, L. M. ; Hozier, J. C. / Hybridization-based karyotyping of mouse chromosomes : Hybridization-bands. In: Cytogenetics and Cell Genetics. 1999 ; Vol. 86, No. 1. pp. 34-38.
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Liechty, MC, Carpio, CM, Aytay, S, Clase, AC, Puschus, KL, Sims, KR, Davis, LM & Hozier, JC 1999, 'Hybridization-based karyotyping of mouse chromosomes: Hybridization-bands', Cytogenetics and Cell Genetics, vol. 86, no. 1, pp. 34-38.

Hybridization-based karyotyping of mouse chromosomes : Hybridization-bands. / Liechty, Melissa C.; Carpio, C. M.; Aytay, S.; Clase, A. C.; Puschus, K. L.; Sims, K. R.; Davis, L. M.; Hozier, J. C.

In: Cytogenetics and Cell Genetics, Vol. 86, No. 1, 14.09.1999, p. 34-38.

Research output: Contribution to journalArticle

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AU - Carpio, C. M.

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AU - Hozier, J. C.

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Liechty MC, Carpio CM, Aytay S, Clase AC, Puschus KL, Sims KR et al. Hybridization-based karyotyping of mouse chromosomes: Hybridization-bands. Cytogenetics and Cell Genetics. 1999 Sep 14;86(1):34-38.