Hydrogen production by Geobacter species and a mixed consortium in a microbial electrolysis cell

Douglas F. Call, Rachel C. Wagner, Bruce E. Logan

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

A hydrogen utilizing exoelectrogenic bacterium (Geobacter sulfurreducens) was compared to both a nonhydrogen oxidizer (Geobacter metallireducens) and a mixed consortium in order to compare the hydrogen production rates and hydrogen recoveries of pure and mixed cultures in microbial electrolysis cells (MECs). At an applied voltage of 0.7 V, both G. sulfurreducens and the mixed culture generated similar current densities (ca. 160 A/m3), resulting in hydrogen production rates of ca. 1.9 m3 H2/m 3/day, whereas G. metallireducens exhibited lower current densities and production rates of 110 ± 7 A/m3 and 1.3 ± 0.1 m3 H2/m3/day, respectively. Before methane was detected in the mixed-culture MEC, the mixed consortium achieved the highest overall energy recovery (relative to both electricity and substrate energy inputs) of 82% ± 8% compared to G. sulfurreducens (77% ± 2%) and G. metallireducens (78% ± 5%), due to the higher coulombic efficiency of the mixed consortium. At an applied voltage of 0.4 V, methane production increased in the mixed-culture MEC and, as a result, the hydrogen recovery decreased and the overall energy recovery dropped to 38% ± 16% compared to 80% ± 5% for G. sulfurreducens and 76% ± 0% for G. metallireducens. Internal hydrogen recycling was confirmed since the mixed culture generated a stable current density of 31 ± 0 A/m3 when fed hydrogen gas, whereas G. sulfurreducens exhibited a steady decrease in current production. Community analysis suggested that G. sulfurreducens was predominant in the mixed-culture MEC (72% of clones) despite its relative absence in the mixed-culture inoculum obtained from a microbial fuel cell reactor (2% of clones). These results demonstrate that Geobacter species are capable of obtaining similar hydrogen production rates and energy recoveries as mixed cultures in an MEC and that high coulombic efficiencies in mixed culture MECs can be attributed in part to the recycling of hydrogen into current.

Original languageEnglish (US)
Pages (from-to)7579-7587
Number of pages9
JournalApplied and environmental microbiology
Volume75
Issue number24
DOIs
StatePublished - Dec 1 2009

Fingerprint

Geobacter
Electrolysis
hydrogen production
mixed culture
Geobacter sulfurreducens
Hydrogen
electrokinesis
Geobacter metallireducens
hydrogen
energy recovery
cells
density current
Methane
Recycling
recycling
clone
Clone Cells
methane
Bioelectric Energy Sources
microbial fuel cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

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title = "Hydrogen production by Geobacter species and a mixed consortium in a microbial electrolysis cell",
abstract = "A hydrogen utilizing exoelectrogenic bacterium (Geobacter sulfurreducens) was compared to both a nonhydrogen oxidizer (Geobacter metallireducens) and a mixed consortium in order to compare the hydrogen production rates and hydrogen recoveries of pure and mixed cultures in microbial electrolysis cells (MECs). At an applied voltage of 0.7 V, both G. sulfurreducens and the mixed culture generated similar current densities (ca. 160 A/m3), resulting in hydrogen production rates of ca. 1.9 m3 H2/m 3/day, whereas G. metallireducens exhibited lower current densities and production rates of 110 ± 7 A/m3 and 1.3 ± 0.1 m3 H2/m3/day, respectively. Before methane was detected in the mixed-culture MEC, the mixed consortium achieved the highest overall energy recovery (relative to both electricity and substrate energy inputs) of 82{\%} ± 8{\%} compared to G. sulfurreducens (77{\%} ± 2{\%}) and G. metallireducens (78{\%} ± 5{\%}), due to the higher coulombic efficiency of the mixed consortium. At an applied voltage of 0.4 V, methane production increased in the mixed-culture MEC and, as a result, the hydrogen recovery decreased and the overall energy recovery dropped to 38{\%} ± 16{\%} compared to 80{\%} ± 5{\%} for G. sulfurreducens and 76{\%} ± 0{\%} for G. metallireducens. Internal hydrogen recycling was confirmed since the mixed culture generated a stable current density of 31 ± 0 A/m3 when fed hydrogen gas, whereas G. sulfurreducens exhibited a steady decrease in current production. Community analysis suggested that G. sulfurreducens was predominant in the mixed-culture MEC (72{\%} of clones) despite its relative absence in the mixed-culture inoculum obtained from a microbial fuel cell reactor (2{\%} of clones). These results demonstrate that Geobacter species are capable of obtaining similar hydrogen production rates and energy recoveries as mixed cultures in an MEC and that high coulombic efficiencies in mixed culture MECs can be attributed in part to the recycling of hydrogen into current.",
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Hydrogen production by Geobacter species and a mixed consortium in a microbial electrolysis cell. / Call, Douglas F.; Wagner, Rachel C.; Logan, Bruce E.

In: Applied and environmental microbiology, Vol. 75, No. 24, 01.12.2009, p. 7579-7587.

Research output: Contribution to journalArticle

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