Hydrogen sulfide oxidation and the arterial chemoreflex

Effect of methemoglobin

Philippe Haouzi, Harold Bell, Maeve Philmon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Endogenous H2S has been proposed to transduce the effects of hypoxia in the carotid bodies (CB). To test this hypothesis, we created a sink for endogenously produced H2S by inducing ~10% methemoglobinemia via the injection of 250mg of sodium nitrite in spontaneously breathing anaesthetized sheep. Methemoglobinemia has been shown to catalyze the oxidation of large quantities of sulfide in the blood and tissues. We found that the presence of metHb completely abolished the ventilatory stimulation induced by 10mg NaHS (i.v.), which in control conditions mimicked the effects of breathing 6-7 tidal volumes of nitrogen, confirming the dramatic increase in the oxidative power of the blood for sulfide. The ventilatory responses to hypoxia (10% O2), nitrogen and hyperoxia were in no way depressed by the metHb. Our results demonstrate that the ventilatory chemoreflex is not depressed in the presence of a high oxidative capacity for sulfide and challenge the view that H2S transduces the effects of hypoxia in the CB.

Original languageEnglish (US)
Pages (from-to)273-283
Number of pages11
JournalRespiratory Physiology and Neurobiology
Volume177
Issue number3
DOIs
StatePublished - Aug 15 2011

Fingerprint

Methemoglobin
Hydrogen Sulfide
Sulfides
Methemoglobinemia
Carotid Body
Respiration
Nitrogen
Sodium Nitrite
Hyperoxia
Tidal Volume
Sheep
Injections
Hypoxia

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

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Hydrogen sulfide oxidation and the arterial chemoreflex : Effect of methemoglobin. / Haouzi, Philippe; Bell, Harold; Philmon, Maeve.

In: Respiratory Physiology and Neurobiology, Vol. 177, No. 3, 15.08.2011, p. 273-283.

Research output: Contribution to journalArticle

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AU - Philmon, Maeve

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