Hydrophobic interactions in the hinge domain of DNA polymerase β are important but not sufficient for maintaining fidelity of DNA synthesis

Patricia L. Opresko, Ross Shiman, Kristin A. Eckert

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We previously described a general mutator form of mammalian DNA polymerase β containing a cysteine substitution for tyrosine 265. Residue 265 localizes to a Hydrophobic hinge region predicted to mediate a polymerase conformational change that may aid in nucleotide selectivity. In this study we tested the hypothesis that van der Waals and hydrophobic contacts between Y265 and neighboring residues are important for DNA synthesis fidelity and catalysis, by altering interactions in the hinge domain via substitution at position 265. Consistent with the importance of hydrophobic interactions, we found that phenylalanine, leucine, and tryptophan substitutions did not alter significantly the steady-state catalytic efficiency of DNA synthesis, relative to wild type, while the polar serine substitution decreased catalytic efficiency 6-fold. However, we found that all substitutions other than phenylalanine increased the error frequency, relative to wild type, in the order serine > tryptophan = leucine. Therefore, maintenance of the hydrophobicity of residue 265 was not sufficient for maintaining fidelity of DNA synthesis. We conclude that while hydrophobic interactions in the hinge domain are important for fidelity, additional factors such as electrostatic and van der Waals interactions contributed by the tyrosine 265 aromatic ring are required to retain wild-type fidelity.

Original languageEnglish (US)
Pages (from-to)11399-11407
Number of pages9
JournalBiochemistry
Volume39
Issue number37
StatePublished - Sep 19 2000

All Science Journal Classification (ASJC) codes

  • Biochemistry

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