Hydroxyproline interference during the gas chromatographic analysis of D/L aspartic acid in human dentine

E. R. Waite, M. J. Collins, Adri Van Duin

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate age at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the NTFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non- collagenous protein fraction.

Original languageEnglish (US)
Pages (from-to)124-131
Number of pages8
JournalInternational Journal of Legal Medicine
Volume112
Issue number2
DOIs
StatePublished - Jan 1 1999

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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