Hypernatremia-induced vasopressin secretion is not altered in TRPV1-/-rats

Andrew Blake Tucker, Sean Stocker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Changes in osmolality or extracellular NaCl concentrations are detected by specialized neurons in the hypothalamus to increase vasopressin (VP) and stimulate thirst. Recent in vitro evidence suggests this process is mediated by an NH2-terminal variant of the transient receptor potential vanilloid type 1 (TRPV1) channel expressed by osmosensitive neurons of the lamina terminalis and vasopressinergic neurons of the supraoptic nucleus. The present study tested this hypothesis in vivo by analysis of plasma VP levels during acute hypernatremia in awake control and TRPV1-/-rats. TRPV1-/-rats were produced by a Zinc-fingernuclease 2-bp deletion in exon 13. Intravenous injection of the TRPV1 agonist capsaicin produced hypotension and bradycardia in control rats, but this response was absent in TRPV1-/-rats. Infusion of 2 M NaCl (1 ml/h iv) increased plasma osmolality, electrolytes, and VP levels in both control and TRPV1-/-rats. However, plasma VP levels did not differ between strains at any time. Furthermore, a linear regression between plasma VP versus osmolality revealed a significant correlation in both control and TRPV1-/-rats, but the slope of the regression lines was not attenuated in TRPV1-/-versus control rats. Hypotension produced by intravenous injection of minoxidil decreased blood pressure and increased plasma VP levels similarly in both groups. Finally, both treatments stimulated thirst; however, cumulative water intakes in response to hypernatremia or hypotension were not different between control and TRPV1-/-rats. These findings suggest that TRPV1 channels are not necessary for VP secretion and thirst stimulated by hypernatremia.

Original languageEnglish (US)
Pages (from-to)R451-R456
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume311
Issue number3
DOIs
StatePublished - Jan 1 2016

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Hypernatremia
Vasopressins
Thirst
Hypotension
Osmolar Concentration
Neurons
Intravenous Injections
Hypothalamus
Minoxidil
Supraoptic Nucleus
Capsaicin
Bradycardia
rat Trpv1 protein
Electrolytes
Drinking
Zinc
Exons
Linear Models
Blood Pressure
vanilloid receptor subtype 1

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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abstract = "Changes in osmolality or extracellular NaCl concentrations are detected by specialized neurons in the hypothalamus to increase vasopressin (VP) and stimulate thirst. Recent in vitro evidence suggests this process is mediated by an NH2-terminal variant of the transient receptor potential vanilloid type 1 (TRPV1) channel expressed by osmosensitive neurons of the lamina terminalis and vasopressinergic neurons of the supraoptic nucleus. The present study tested this hypothesis in vivo by analysis of plasma VP levels during acute hypernatremia in awake control and TRPV1-/-rats. TRPV1-/-rats were produced by a Zinc-fingernuclease 2-bp deletion in exon 13. Intravenous injection of the TRPV1 agonist capsaicin produced hypotension and bradycardia in control rats, but this response was absent in TRPV1-/-rats. Infusion of 2 M NaCl (1 ml/h iv) increased plasma osmolality, electrolytes, and VP levels in both control and TRPV1-/-rats. However, plasma VP levels did not differ between strains at any time. Furthermore, a linear regression between plasma VP versus osmolality revealed a significant correlation in both control and TRPV1-/-rats, but the slope of the regression lines was not attenuated in TRPV1-/-versus control rats. Hypotension produced by intravenous injection of minoxidil decreased blood pressure and increased plasma VP levels similarly in both groups. Finally, both treatments stimulated thirst; however, cumulative water intakes in response to hypernatremia or hypotension were not different between control and TRPV1-/-rats. These findings suggest that TRPV1 channels are not necessary for VP secretion and thirst stimulated by hypernatremia.",
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Hypernatremia-induced vasopressin secretion is not altered in TRPV1-/-rats. / Tucker, Andrew Blake; Stocker, Sean.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 311, No. 3, 01.01.2016, p. R451-R456.

Research output: Contribution to journalArticle

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