Identification and characterisation of new Campylobacter group III phages of animal origin

Nika Janež, Andreja Kokošin, Eva Zaletel, Tanja Vranac, Jasna Kovac, Darinka Vučković, Sonja Smole Možina, Vladka Curin Serbec, Qijing Zhang, Tomaž Accetto, Aleš Podgornik, Matjaž Peterka

Research output: Contribution to journalLetter

11 Citations (Scopus)

Abstract

Campylobacter-specific bacteriophages (phages) are considered as an alternative intervention strategy to decrease the level of poultry contamination with Campylobacter, a leading cause of gastroenteritis worldwide. Eradication efficiency depends primarily on phage-host interaction mediated by phage tail-spike proteins and bacterial receptors. Here, this interaction was characterised using tailspike gene sequence analysis, phage neutralisation by antiserum and host range analysis of newly isolated group III Campylobacter phages with 68 Campylobacter jejuni and Campylobacter coli strains. Three different groups of phages were obtained using antibody neutralisation assay, and they were further divided according to polymorphisms observed within tail fibre sequences and host range. Only moderate congruence was observed between these criteria with notable exception of two phages. The infection relied on capsule in all phages isolated, and flagella were found to influence phage propagation on agar plates, but not in broth. Their specificity was more C. jejuni oriented with tendency to lyse human isolates more efficiently. Additionally, natural resistance of C. jejuni to phages did not correlate with their antibiotic resistance patterns. These findings provide new insights into Campylobacter-phage interaction.

Original languageEnglish (US)
Pages (from-to)64-71
Number of pages8
JournalFEMS Microbiology Letters
Volume359
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Campylobacter
Bacteriophages
Campylobacter jejuni
Host Specificity
Tail
Campylobacter coli
Bacterial Proteins
Flagella
Gastroenteritis
Poultry
Microbial Drug Resistance
Innate Immunity
Agar
Capsules
Sequence Analysis
Immune Sera

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Janež, N., Kokošin, A., Zaletel, E., Vranac, T., Kovac, J., Vučković, D., ... Peterka, M. (2014). Identification and characterisation of new Campylobacter group III phages of animal origin. FEMS Microbiology Letters, 359(1), 64-71. https://doi.org/10.1111/1574-6968.12556
Janež, Nika ; Kokošin, Andreja ; Zaletel, Eva ; Vranac, Tanja ; Kovac, Jasna ; Vučković, Darinka ; Možina, Sonja Smole ; Serbec, Vladka Curin ; Zhang, Qijing ; Accetto, Tomaž ; Podgornik, Aleš ; Peterka, Matjaž. / Identification and characterisation of new Campylobacter group III phages of animal origin. In: FEMS Microbiology Letters. 2014 ; Vol. 359, No. 1. pp. 64-71.
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Janež, N, Kokošin, A, Zaletel, E, Vranac, T, Kovac, J, Vučković, D, Možina, SS, Serbec, VC, Zhang, Q, Accetto, T, Podgornik, A & Peterka, M 2014, 'Identification and characterisation of new Campylobacter group III phages of animal origin', FEMS Microbiology Letters, vol. 359, no. 1, pp. 64-71. https://doi.org/10.1111/1574-6968.12556

Identification and characterisation of new Campylobacter group III phages of animal origin. / Janež, Nika; Kokošin, Andreja; Zaletel, Eva; Vranac, Tanja; Kovac, Jasna; Vučković, Darinka; Možina, Sonja Smole; Serbec, Vladka Curin; Zhang, Qijing; Accetto, Tomaž; Podgornik, Aleš; Peterka, Matjaž.

In: FEMS Microbiology Letters, Vol. 359, No. 1, 01.01.2014, p. 64-71.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Identification and characterisation of new Campylobacter group III phages of animal origin

AU - Janež, Nika

AU - Kokošin, Andreja

AU - Zaletel, Eva

AU - Vranac, Tanja

AU - Kovac, Jasna

AU - Vučković, Darinka

AU - Možina, Sonja Smole

AU - Serbec, Vladka Curin

AU - Zhang, Qijing

AU - Accetto, Tomaž

AU - Podgornik, Aleš

AU - Peterka, Matjaž

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Campylobacter-specific bacteriophages (phages) are considered as an alternative intervention strategy to decrease the level of poultry contamination with Campylobacter, a leading cause of gastroenteritis worldwide. Eradication efficiency depends primarily on phage-host interaction mediated by phage tail-spike proteins and bacterial receptors. Here, this interaction was characterised using tailspike gene sequence analysis, phage neutralisation by antiserum and host range analysis of newly isolated group III Campylobacter phages with 68 Campylobacter jejuni and Campylobacter coli strains. Three different groups of phages were obtained using antibody neutralisation assay, and they were further divided according to polymorphisms observed within tail fibre sequences and host range. Only moderate congruence was observed between these criteria with notable exception of two phages. The infection relied on capsule in all phages isolated, and flagella were found to influence phage propagation on agar plates, but not in broth. Their specificity was more C. jejuni oriented with tendency to lyse human isolates more efficiently. Additionally, natural resistance of C. jejuni to phages did not correlate with their antibiotic resistance patterns. These findings provide new insights into Campylobacter-phage interaction.

AB - Campylobacter-specific bacteriophages (phages) are considered as an alternative intervention strategy to decrease the level of poultry contamination with Campylobacter, a leading cause of gastroenteritis worldwide. Eradication efficiency depends primarily on phage-host interaction mediated by phage tail-spike proteins and bacterial receptors. Here, this interaction was characterised using tailspike gene sequence analysis, phage neutralisation by antiserum and host range analysis of newly isolated group III Campylobacter phages with 68 Campylobacter jejuni and Campylobacter coli strains. Three different groups of phages were obtained using antibody neutralisation assay, and they were further divided according to polymorphisms observed within tail fibre sequences and host range. Only moderate congruence was observed between these criteria with notable exception of two phages. The infection relied on capsule in all phages isolated, and flagella were found to influence phage propagation on agar plates, but not in broth. Their specificity was more C. jejuni oriented with tendency to lyse human isolates more efficiently. Additionally, natural resistance of C. jejuni to phages did not correlate with their antibiotic resistance patterns. These findings provide new insights into Campylobacter-phage interaction.

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U2 - 10.1111/1574-6968.12556

DO - 10.1111/1574-6968.12556

M3 - Letter

VL - 359

SP - 64

EP - 71

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