Identification and characterization of ζ-opioid receptor in human colon cancer

Staci D. Hytrek, Jill P. Smith, Thomas J. McGarrity, Patricia J. McLaughlin, C. Max Lang, Lan S. Zagon

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Opioid growth factor (OGF, [Met5]enkephalin) inhibits the growth of human colon cancer in nude mice in a receptor-mediated fashion. Ligand binding assays using HT-29 human colon cancer tissue and [3H][Met5]enkephalin were performed to characterize the receptor responsible for the growth-regulatory effects of OGF in colon cancer. Specific and saturable binding was detected, and Scatchard analysis revealed that the data were consistent for a single binding site with a binding affinity of 15.4 ± 2.0 nM and a binding capacity of 364.8 ± 25.7 fmol/mg protein. Subcellular fractionation studies revealed that binding was restricted to the nuclear fraction. Competition experiments showed that cold [Met5]enkephalin was the most effective ligand at displacing [3H][Met5]enkephalin. Binding to radiolabeled [Met5]enkephalin also was detected in colon cancers obtained from surgical resections. The function, pharmacological and biochemical characteristics, distribution, and subcellular location of this OGF receptor in human colon cancer are consistent with the ζ-opioid receptor.

Original languageEnglish (US)
Pages (from-to)R115-R121
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume271
Issue number1 40-1
StatePublished - Jul 1 1996

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Identification and characterization of ζ-opioid receptor in human colon cancer'. Together they form a unique fingerprint.

  • Cite this