Identification and characterization of a cellulose binding heptapeptide revealed by phage display

Jing Guo, Jeffrey M. Catchmark, Mohamed Naseer Ali Mohamed, Alan James Benesi, Ming Tien, Teh-hui Kao, Heath D. Watts, James D. Kubicki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Cellulose nanowhiskers (CNWs) were used in conjunction with phage display technology to identify polypeptides which bind the crystalline region of cellulose. A consensus peptide WHWTYYW was identified to efficiently bind the CNWs. The binding affinities of specific phage particles were assessed using biopanning assays and enzyme-linked immunosorbent assay (ELISA). The WHWTYYW peptide was synthesized and isothermal titration calorimetry (ITC) analysis showed that the peptide exhibited a binding constant of ∼105 M-1 toward the crystalline CNWs. In order to understand how the affinity of this peptide differs for noncrystalline cellulose, binding properties were characterized using cello-oligosaccharides as substrates. Binding analysis was performed using UV spectroscopy and fluorescence quenching experiments. The specific molecular interactions of the WHWTYYW peptide with cellohexaose were examined using nuclear magnetic resonance (NMR). Interactions of this peptide with crystalline cellulose were also investigated using classical molecular modeling and quantum mechanical calculations of 13C NMR chemical shifts. The NMR experiments and calculations indicate that the WHWTYYW peptide exhibits a bent structure when bound, allowing the Y5 amino acid to form a CH/π stacking interaction and H-bond with the glucose ring of cellulose.

Original languageEnglish (US)
Pages (from-to)1795-1805
Number of pages11
JournalBiomacromolecules
Volume14
Issue number6
DOIs
StatePublished - Jun 10 2013

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Bacteriophages
Cellulose
Peptides
Display devices
Nanowhiskers
Nuclear magnetic resonance
Crystalline materials
Assays
Oligosaccharides
Molecular modeling
Molecular interactions
Polypeptides
Immunosorbents
Chemical shift
Calorimetry
Ultraviolet spectroscopy
Titration
Glucose
Amino acids
Quenching

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Guo, Jing ; Catchmark, Jeffrey M. ; Mohamed, Mohamed Naseer Ali ; Benesi, Alan James ; Tien, Ming ; Kao, Teh-hui ; Watts, Heath D. ; Kubicki, James D. / Identification and characterization of a cellulose binding heptapeptide revealed by phage display. In: Biomacromolecules. 2013 ; Vol. 14, No. 6. pp. 1795-1805.
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Identification and characterization of a cellulose binding heptapeptide revealed by phage display. / Guo, Jing; Catchmark, Jeffrey M.; Mohamed, Mohamed Naseer Ali; Benesi, Alan James; Tien, Ming; Kao, Teh-hui; Watts, Heath D.; Kubicki, James D.

In: Biomacromolecules, Vol. 14, No. 6, 10.06.2013, p. 1795-1805.

Research output: Contribution to journalArticle

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