Identification and characterization of a TAP-family gene in the lamprey

Tatiana S. Uinuk-Ool, Werner E. Mayer, Akie Sato, Naoko Takezaki, Lesley Benyon, Max D. Cooper, Jan Klein

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

An expressed sequence tag obtained from a sea lamprey (Petromyzon marinus) cDNA library was used to obtain a full-length coding sequence showing significant similarity to ABCB transporter proteins. The sequence is closely related to the mammalian ABCB9 protein and the TAP1 and TAP2 proteins that transport peptides for loading onto nascent Mhc class I molecules. The Pema-ABCB9 gene has an exon-intron organization similar to that of the mammalian TAP genes, with the exception of exon 2, which in the lamprey is split into two by a 949-bp long intron. The gene probably occurs in a single copy in the haploid lamprey genome. The ABCB9 genes appear to be evolving four-to-ten times slower than the TAP1 and TAP2 genes. Six putative transmembrane helices and the nucleotide-binding domain of the lamprey ABCB9 protein show high sequence similarity with the TAP1 and TAP2 molecules. The lamprey protein also contains sequence stretches that resemble the putative peptide interacting parts of the TAP1 and TAP2 molecules, but are peppered with ABCB9-specific residues.

Original languageEnglish (US)
Pages (from-to)38-48
Number of pages11
JournalImmunogenetics
Volume55
Issue number1
StatePublished - Apr 1 2003

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Lampreys
Petromyzon
Genes
Introns
Exons
Peptides
Proteins
Haploidy
Expressed Sequence Tags
Protein Transport
Gene Library
Nucleotides
Genome

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Uinuk-Ool, T. S., Mayer, W. E., Sato, A., Takezaki, N., Benyon, L., Cooper, M. D., & Klein, J. (2003). Identification and characterization of a TAP-family gene in the lamprey. Immunogenetics, 55(1), 38-48.
Uinuk-Ool, Tatiana S. ; Mayer, Werner E. ; Sato, Akie ; Takezaki, Naoko ; Benyon, Lesley ; Cooper, Max D. ; Klein, Jan. / Identification and characterization of a TAP-family gene in the lamprey. In: Immunogenetics. 2003 ; Vol. 55, No. 1. pp. 38-48.
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Uinuk-Ool, TS, Mayer, WE, Sato, A, Takezaki, N, Benyon, L, Cooper, MD & Klein, J 2003, 'Identification and characterization of a TAP-family gene in the lamprey', Immunogenetics, vol. 55, no. 1, pp. 38-48.

Identification and characterization of a TAP-family gene in the lamprey. / Uinuk-Ool, Tatiana S.; Mayer, Werner E.; Sato, Akie; Takezaki, Naoko; Benyon, Lesley; Cooper, Max D.; Klein, Jan.

In: Immunogenetics, Vol. 55, No. 1, 01.04.2003, p. 38-48.

Research output: Contribution to journalArticle

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Uinuk-Ool TS, Mayer WE, Sato A, Takezaki N, Benyon L, Cooper MD et al. Identification and characterization of a TAP-family gene in the lamprey. Immunogenetics. 2003 Apr 1;55(1):38-48.