Identification and characterization of peanut stunt cucumovirus from naturally infected alfalfa in Iran

K. Bananej, M. R. Hajimorad, Marilyn J. Roossinck, N. Shahraeen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A mechanically transmissible virus was isolated from a naturally infected alfalfa plant (Medicago satira) in Karaj, Iran. It induced fern-leaf symptoms in Lycopersicon esculentum, general chlorosis and stunting in Arachis hypogoea, local lesions and systemic infection in Chenopodium quinoa, Phaseolus vulgaris and Vigna unguiculata but only local lesions in C. amaranticolor. In gel-immunodiffusion tests, it reacted strongly with an antiserum to peanut stunt cucumovirus (PSV), moderately with two of six antisera to cucumber mosaic cucumovirus (CMV) and with two of five antisera to tomato aspermy cucumovirus. Its spherical virions (28 nm in diameter) contained a coat protein of approximately 29 kDa and encapsidated four species of RNAs with similar electrophoretic mobilities to RNAs 1-4 of CMV strains Fny and LS and those of PSV strains J and W. Its encapsidated RNAs hybridized in slot-blot hybridization assay with the complementary DNA probe of PSV-W RNAs but not with those of CMV strains. Therefore, on the basis of biological, serological and physico-biochemical properties, the virus was identified as PSV. No satellite RNA was associated with the virus. This is the first report of PSV in Iran.

Original languageEnglish (US)
Pages (from-to)355-361
Number of pages7
JournalPlant Pathology
Volume47
Issue number3
DOIs
StatePublished - Jan 1 1998

Fingerprint

Peanut stunt virus
Cucumovirus
Medicago sativa
Iran
alfalfa
RNA
antiserum
lesions (plant)
viruses
Tomato aspermy virus
satellite RNA
Chenopodium giganteum
Immune Sera
Chenopodium quinoa
Arachis
immunodiffusion tests
complementary DNA
Medicago
Cucumber mosaic virus
Lycopersicon esculentum

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Cite this

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abstract = "A mechanically transmissible virus was isolated from a naturally infected alfalfa plant (Medicago satira) in Karaj, Iran. It induced fern-leaf symptoms in Lycopersicon esculentum, general chlorosis and stunting in Arachis hypogoea, local lesions and systemic infection in Chenopodium quinoa, Phaseolus vulgaris and Vigna unguiculata but only local lesions in C. amaranticolor. In gel-immunodiffusion tests, it reacted strongly with an antiserum to peanut stunt cucumovirus (PSV), moderately with two of six antisera to cucumber mosaic cucumovirus (CMV) and with two of five antisera to tomato aspermy cucumovirus. Its spherical virions (28 nm in diameter) contained a coat protein of approximately 29 kDa and encapsidated four species of RNAs with similar electrophoretic mobilities to RNAs 1-4 of CMV strains Fny and LS and those of PSV strains J and W. Its encapsidated RNAs hybridized in slot-blot hybridization assay with the complementary DNA probe of PSV-W RNAs but not with those of CMV strains. Therefore, on the basis of biological, serological and physico-biochemical properties, the virus was identified as PSV. No satellite RNA was associated with the virus. This is the first report of PSV in Iran.",
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Identification and characterization of peanut stunt cucumovirus from naturally infected alfalfa in Iran. / Bananej, K.; Hajimorad, M. R.; Roossinck, Marilyn J.; Shahraeen, N.

In: Plant Pathology, Vol. 47, No. 3, 01.01.1998, p. 355-361.

Research output: Contribution to journalArticle

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