Identification and distinct regulation of yeast TATA box-containing genes

Andrew D. Basehoar; Sara J. Zanton; B. Franklin Pugh

doi:10.1016/S0092-8674(04)00205-3

Identification and distinct regulation of yeast TATA box-containing genes

Andrew D. Basehoar, Sara J. Zanton, B. Franklin Pugh

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493 Scopus citations

Abstract

Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are associated with responses to stress, are highly regulated, and preferentially utilize SAGA rather than TFIID when compared to TATA-less promoters. Transcriptional regulation in yeast appears to be mechanistically bipolar, possibly reflecting a need to balance inducible stress-related responses with constitutive housekeeping functions.

Original language	English (US)
Pages (from-to)	699-709
Number of pages	11
Journal	Cell
Volume	116
Issue number	5
DOIs	https://doi.org/10.1016/S0092-8674(04)00205-3
State	Published - Mar 5 2004

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/S0092-8674(04)00205-3

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title = "Identification and distinct regulation of yeast TATA box-containing genes",

abstract = "Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are associated with responses to stress, are highly regulated, and preferentially utilize SAGA rather than TFIID when compared to TATA-less promoters. Transcriptional regulation in yeast appears to be mechanistically bipolar, possibly reflecting a need to balance inducible stress-related responses with constitutive housekeeping functions.",

author = "Basehoar, {Andrew D.} and Zanton, {Sara J.} and Pugh, {B. Franklin}",

note = "Funding Information: We thank Naomi Altman, Francesca Chiaromonte, and Kathryn Huisinga for invaluable advice. We thank the Simpson and Workman laboratories for generating and providing the intergenic microarrays. This work was supported by NIH grant GM059055. ",

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T1 - Identification and distinct regulation of yeast TATA box-containing genes

AU - Basehoar, Andrew D.

AU - Zanton, Sara J.

AU - Pugh, B. Franklin

N1 - Funding Information: We thank Naomi Altman, Francesca Chiaromonte, and Kathryn Huisinga for invaluable advice. We thank the Simpson and Workman laboratories for generating and providing the intergenic microarrays. This work was supported by NIH grant GM059055.

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N2 - Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are associated with responses to stress, are highly regulated, and preferentially utilize SAGA rather than TFIID when compared to TATA-less promoters. Transcriptional regulation in yeast appears to be mechanistically bipolar, possibly reflecting a need to balance inducible stress-related responses with constitutive housekeeping functions.

AB - Despite being one of the first eukaryotic transcriptional regulatory elements identified, the sequence of a native TATA box and its significance remain elusive. Applying criteria associated with TATA boxes we queried several Saccharomyces genomes and arrived at the consensus TATA(A/T)A(A/T)(A/G). Approximately 20% of yeast genes contain a TATA box. Strikingly, TATA box-containing genes are associated with responses to stress, are highly regulated, and preferentially utilize SAGA rather than TFIID when compared to TATA-less promoters. Transcriptional regulation in yeast appears to be mechanistically bipolar, possibly reflecting a need to balance inducible stress-related responses with constitutive housekeeping functions.

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Identification and distinct regulation of yeast TATA box-containing genes

Abstract

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