Identification and linkage of the proteasome activator complex PA28 subunit genes in zebrafish

B. W. Murray, H. Sültmann, Jan Klein

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

PA28 is an activator of the latent 20S proteasome, a large multisubunit complex involved in intracellular proteolysis. Two forms of hexameric PA28 have been identified, PA28-(αβ)3 and PA28-(γ)6, of which the former is of immunological importance. Both the PA28-α and PA28-β subunits are inducible by interferon-γ (IFN-γ) and the PA28-(αβ)3 complex enhances the ability of the 20S proteasome to produce peptides suited for binding to major histocompatibility complex (Mhc) class I molecules. To identify the homologues of the PA28 subunits in zebrafish we screened a cDNA library and obtained full-length cDNA sequences of the genes PSME1, PSME2 and PSME3 coding for the PA28-α, PA28-β and PA28-γ subunits, respectively. Phylogenetic analysis indicates the existence of the ancestors of all three genes prior to the divergence of tetrapods and bony fishes. The IFN-γ- inducible subunits, PA28-α and PA28-β, evolve faster than the presumably older PA28-γ subunit. Using zebrafish radiation hybrid panels, the genes PSME2 and PSME3 were mapped to linkage group 12 and shown to be separated by a distance of less than 2.4 cm. This observation suggests that an intrachromosomal duplication event created the precursor of the IFN-γ- inducible genes from a PA28-γ-like ancestor prior to their recruitment into the Mhc class I peptide presentation pathway.

Original languageEnglish (US)
Pages (from-to)571-576
Number of pages6
JournalScandinavian Journal of Immunology
Volume51
Issue number6
DOIs
StatePublished - Jun 26 2000

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Zebrafish
Proteasome Endopeptidase Complex
Interferons
Major Histocompatibility Complex
Genes
Radiation Hybrid Mapping
Peptides
Gene Library
Proteolysis
Fishes
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Murray, B. W. ; Sültmann, H. ; Klein, Jan. / Identification and linkage of the proteasome activator complex PA28 subunit genes in zebrafish. In: Scandinavian Journal of Immunology. 2000 ; Vol. 51, No. 6. pp. 571-576.
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Identification and linkage of the proteasome activator complex PA28 subunit genes in zebrafish. / Murray, B. W.; Sültmann, H.; Klein, Jan.

In: Scandinavian Journal of Immunology, Vol. 51, No. 6, 26.06.2000, p. 571-576.

Research output: Contribution to journalArticle

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