Identification and mapping of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora

M. Malnoy, E. E. Borejsza-Wysocka, H. S. Aldwinckle, A. M. Baldo, C. M. Carlisle, D. Bowatte, S. E. Gardiner, J. L. Norelli, C. L. Bassett, R. E. Farrell

Research output: Contribution to journalConference article

2 Citations (Scopus)

Abstract

The necrogenic enterobacterium, Erwinia amylovora (Ea) is the causal agent of the fire blight (FB) disease of many Rosaceae species, including apple and pear. During the infection process, the bacteria induce an oxidative stress response with kinetics similar to those induced in an incompatible bacteria-plant interaction. In order to understand the mechanisms that distinguish resistant and susceptible responses in FB resistant and susceptible apple genotypes after inoculation with Ea, differentially expressed genes were identified by cDNA-AFLP analysis. cDNA was isolated from M.26 (susceptible) and G.41 (resistant) apple tissues collected 2 and 48 h after challenge with a virulent Ea strain or mock (buffer) inoculated. To identify differentially expressed transcripts, electrophoretic banding patterns obtained from cDNAs treated with the Licor cDNA-AFLP kit. In the AFLP experiments, using both kits, M.26 and G.41 showed different patterns of expression, including genes specifically induced, not induced or repressed by Ea. In total 190 EST's differentially expressed between M.26 and G41 were identified using 42 pairs of AFLP primers. cDNA-AFLP analysis of global EST expression in a resistant and a susceptible apple genotype identified three major classes of genes. Sequencing data for the EST's showed that genes linked to resistance, encoding proteins involved in perception, signaling, defense and apoptosis were modulated by Ea in its host plant. The time course of expression of some of these EST's selected via a bioinformatic analysis has been determined. Some of these EST's have also been mapped.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalActa Horticulturae
Volume793
StatePublished - Dec 1 2008
Event11th International Workshop on Fire Blight - Portland, OR, United States
Duration: Aug 12 2007Aug 17 2007

Fingerprint

Erwinia amylovora
Malus domestica
chromosome mapping
apples
amplified fragment length polymorphism
cultivars
genes
genotype
bacteria
Rosaceae
bioinformatics
pears
stress response
oxidative stress
host plants
buffers
apoptosis
kinetics
gene expression
infection

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Malnoy, M. ; Borejsza-Wysocka, E. E. ; Aldwinckle, H. S. ; Baldo, A. M. ; Carlisle, C. M. ; Bowatte, D. ; Gardiner, S. E. ; Norelli, J. L. ; Bassett, C. L. ; Farrell, R. E. / Identification and mapping of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora. In: Acta Horticulturae. 2008 ; Vol. 793. pp. 195-200.
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abstract = "The necrogenic enterobacterium, Erwinia amylovora (Ea) is the causal agent of the fire blight (FB) disease of many Rosaceae species, including apple and pear. During the infection process, the bacteria induce an oxidative stress response with kinetics similar to those induced in an incompatible bacteria-plant interaction. In order to understand the mechanisms that distinguish resistant and susceptible responses in FB resistant and susceptible apple genotypes after inoculation with Ea, differentially expressed genes were identified by cDNA-AFLP analysis. cDNA was isolated from M.26 (susceptible) and G.41 (resistant) apple tissues collected 2 and 48 h after challenge with a virulent Ea strain or mock (buffer) inoculated. To identify differentially expressed transcripts, electrophoretic banding patterns obtained from cDNAs treated with the Licor cDNA-AFLP kit. In the AFLP experiments, using both kits, M.26 and G.41 showed different patterns of expression, including genes specifically induced, not induced or repressed by Ea. In total 190 EST's differentially expressed between M.26 and G41 were identified using 42 pairs of AFLP primers. cDNA-AFLP analysis of global EST expression in a resistant and a susceptible apple genotype identified three major classes of genes. Sequencing data for the EST's showed that genes linked to resistance, encoding proteins involved in perception, signaling, defense and apoptosis were modulated by Ea in its host plant. The time course of expression of some of these EST's selected via a bioinformatic analysis has been determined. Some of these EST's have also been mapped.",
author = "M. Malnoy and Borejsza-Wysocka, {E. E.} and Aldwinckle, {H. S.} and Baldo, {A. M.} and Carlisle, {C. M.} and D. Bowatte and Gardiner, {S. E.} and Norelli, {J. L.} and Bassett, {C. L.} and Farrell, {R. E.}",
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Malnoy, M, Borejsza-Wysocka, EE, Aldwinckle, HS, Baldo, AM, Carlisle, CM, Bowatte, D, Gardiner, SE, Norelli, JL, Bassett, CL & Farrell, RE 2008, 'Identification and mapping of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora', Acta Horticulturae, vol. 793, pp. 195-200.

Identification and mapping of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora. / Malnoy, M.; Borejsza-Wysocka, E. E.; Aldwinckle, H. S.; Baldo, A. M.; Carlisle, C. M.; Bowatte, D.; Gardiner, S. E.; Norelli, J. L.; Bassett, C. L.; Farrell, R. E.

In: Acta Horticulturae, Vol. 793, 01.12.2008, p. 195-200.

Research output: Contribution to journalConference article

TY - JOUR

T1 - Identification and mapping of genes differentially expressed during interaction of resistant and susceptible apple cultivars (Malus × domestica) with Erwinia amylovora

AU - Malnoy, M.

AU - Borejsza-Wysocka, E. E.

AU - Aldwinckle, H. S.

AU - Baldo, A. M.

AU - Carlisle, C. M.

AU - Bowatte, D.

AU - Gardiner, S. E.

AU - Norelli, J. L.

AU - Bassett, C. L.

AU - Farrell, R. E.

PY - 2008/12/1

Y1 - 2008/12/1

N2 - The necrogenic enterobacterium, Erwinia amylovora (Ea) is the causal agent of the fire blight (FB) disease of many Rosaceae species, including apple and pear. During the infection process, the bacteria induce an oxidative stress response with kinetics similar to those induced in an incompatible bacteria-plant interaction. In order to understand the mechanisms that distinguish resistant and susceptible responses in FB resistant and susceptible apple genotypes after inoculation with Ea, differentially expressed genes were identified by cDNA-AFLP analysis. cDNA was isolated from M.26 (susceptible) and G.41 (resistant) apple tissues collected 2 and 48 h after challenge with a virulent Ea strain or mock (buffer) inoculated. To identify differentially expressed transcripts, electrophoretic banding patterns obtained from cDNAs treated with the Licor cDNA-AFLP kit. In the AFLP experiments, using both kits, M.26 and G.41 showed different patterns of expression, including genes specifically induced, not induced or repressed by Ea. In total 190 EST's differentially expressed between M.26 and G41 were identified using 42 pairs of AFLP primers. cDNA-AFLP analysis of global EST expression in a resistant and a susceptible apple genotype identified three major classes of genes. Sequencing data for the EST's showed that genes linked to resistance, encoding proteins involved in perception, signaling, defense and apoptosis were modulated by Ea in its host plant. The time course of expression of some of these EST's selected via a bioinformatic analysis has been determined. Some of these EST's have also been mapped.

AB - The necrogenic enterobacterium, Erwinia amylovora (Ea) is the causal agent of the fire blight (FB) disease of many Rosaceae species, including apple and pear. During the infection process, the bacteria induce an oxidative stress response with kinetics similar to those induced in an incompatible bacteria-plant interaction. In order to understand the mechanisms that distinguish resistant and susceptible responses in FB resistant and susceptible apple genotypes after inoculation with Ea, differentially expressed genes were identified by cDNA-AFLP analysis. cDNA was isolated from M.26 (susceptible) and G.41 (resistant) apple tissues collected 2 and 48 h after challenge with a virulent Ea strain or mock (buffer) inoculated. To identify differentially expressed transcripts, electrophoretic banding patterns obtained from cDNAs treated with the Licor cDNA-AFLP kit. In the AFLP experiments, using both kits, M.26 and G.41 showed different patterns of expression, including genes specifically induced, not induced or repressed by Ea. In total 190 EST's differentially expressed between M.26 and G41 were identified using 42 pairs of AFLP primers. cDNA-AFLP analysis of global EST expression in a resistant and a susceptible apple genotype identified three major classes of genes. Sequencing data for the EST's showed that genes linked to resistance, encoding proteins involved in perception, signaling, defense and apoptosis were modulated by Ea in its host plant. The time course of expression of some of these EST's selected via a bioinformatic analysis has been determined. Some of these EST's have also been mapped.

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M3 - Conference article

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