TY - JOUR
T1 - Identification and mapping of late blight resistance quantitative trait loci in tomato accession PI 163245
AU - Ohlson, Erik W.
AU - Ashrafi, Hamid
AU - Foolad, Majid R.
N1 - Funding Information:
The authors graciously thank PhD candidates Mengyuan (Maggie) Jia and Sihui (Olivia) Gao for reviewing the manuscript and making useful comments and suggestions before submission. The authors also thank all Penn State staff, graduate, and undergraduate students who helped with these experiments; Dr. John (Jay) Scott (University of Florida) for providing seed of Fla. 8059; Dr. Randolph Gardner (NC State University) for providing seeds of the control genotypes; the USDA PGRU for providing the original seed for PI 163245; and SolCAP for sequencing the parental lines for GBS analysis. This research was supported in part by the Pennsylvania Vegetable Marketing and Research Program, Mid-America Food Processors Association, the College of Agricultural Sciences, and Department of Plant Science at The Pennsylvania State University.
Publisher Copyright:
© Crop Science Society of America.
PY - 2018
Y1 - 2018
N2 - Late blight (LB), caused by the oomycete Phytophthora infestans (Mont.) de Bary, is one of the most devastating diseases of tomato (Solanum lycopersicum L.) and potato (S. tuberosum L.) worldwide. The importance of LB on tomato has increased due to the occurrence of aggressive and fungicide-resistant clonal lineages of P. infestans. Consequently, identification and characterization of new sources of genetic resistance to LB has become a priority in tomato breeding. Previously, we reported accession PI 163245 as a promising source of highly heritable LB resistance for tomato breeding. The purpose of this study was to identify and map quantitative trait loci (QTLs) associated with LB resistance in this accession using a trait-based marker analysis (a.k.a. selective genotyping). An F2 mapping population (n = 560) derived from a cross between a LB-susceptible tomato breeding line (Fla. 8059) and PI 163245 was screened for LB resistance, and the most resistant (n = 39) and susceptible (n = 35) individuals were selected for genotyping. Sequencing and comparison of the reduced representation libraries (RRLs) derived from genomic DNA of the two parents resulted in the identification of 33,541 putative single nucleotide polymorphism (SNP) markers, of which, 233 genome-wide markers were used to genotype the 74 selected F2 individuals. The marker analysis resulted in the identification of four LB resistance QTLs conferred by PI 163245, located on chromosomes 2, 3, 10, and 11. Research is underway to develop near-isogenic lines (NILs) for fine mapping the QTLs and develop tomato breeding lines with LB resistance introduced from PI 163245.
AB - Late blight (LB), caused by the oomycete Phytophthora infestans (Mont.) de Bary, is one of the most devastating diseases of tomato (Solanum lycopersicum L.) and potato (S. tuberosum L.) worldwide. The importance of LB on tomato has increased due to the occurrence of aggressive and fungicide-resistant clonal lineages of P. infestans. Consequently, identification and characterization of new sources of genetic resistance to LB has become a priority in tomato breeding. Previously, we reported accession PI 163245 as a promising source of highly heritable LB resistance for tomato breeding. The purpose of this study was to identify and map quantitative trait loci (QTLs) associated with LB resistance in this accession using a trait-based marker analysis (a.k.a. selective genotyping). An F2 mapping population (n = 560) derived from a cross between a LB-susceptible tomato breeding line (Fla. 8059) and PI 163245 was screened for LB resistance, and the most resistant (n = 39) and susceptible (n = 35) individuals were selected for genotyping. Sequencing and comparison of the reduced representation libraries (RRLs) derived from genomic DNA of the two parents resulted in the identification of 33,541 putative single nucleotide polymorphism (SNP) markers, of which, 233 genome-wide markers were used to genotype the 74 selected F2 individuals. The marker analysis resulted in the identification of four LB resistance QTLs conferred by PI 163245, located on chromosomes 2, 3, 10, and 11. Research is underway to develop near-isogenic lines (NILs) for fine mapping the QTLs and develop tomato breeding lines with LB resistance introduced from PI 163245.
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U2 - 10.3835/plantgenome2018.01.0007
DO - 10.3835/plantgenome2018.01.0007
M3 - Article
C2 - 30512045
AN - SCOPUS:85057346301
VL - 11
JO - Plant Genome
JF - Plant Genome
SN - 1940-3372
M1 - 180007
ER -