Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2μ circle

James Broach, John F. Atkins, Carolyn McGill, Louise Chow

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have identified two major and approximately ten minor poly(A)-containing RNA species in S. cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2μ circle. The two major species, which are 1325 and 1275 bases in length, are transcribed from the two unique halves of the plasmid and extend into the inverted repeat sequences which separate the unique regions. The map positions of the minor transcripts, which range in length from 350 to 2600 bases, indicate that except for a small region of the genome in which no transcription is observed, both strands of the entire 2μ circle genome are transcribed. We also present evidence demonstrating that RNA transcribed from 2μ circular DNA is used to program the synthesis of specific proteins in yeast: that is, yeast RNA complementary to 2μ circle DNA can be translated in vitro to produce specific polypeptides of substantial size. Finally, the pattern of transcription of 2μ circle suggests the possibility that messenger RNA species are derived by cleavage of larger transcripts, and in addition, that the intramolecular recombination of 2μ circle which occurs in yeast functions as a genetic switch to allow separate expression of two sets of genes on the 2μ circle genome.

Original languageEnglish (US)
Pages (from-to)827-839
Number of pages13
JournalCell
Volume16
Issue number4
DOIs
StatePublished - Jan 1 1979

Fingerprint

Yeast
Plasmids
Transcription
Genes
Yeasts
Genome
Inverted Repeat Sequences
Circular DNA
Complementary RNA
Messenger RNA
Fungal Proteins
RNA
Genetic Recombination
Poly A
Saccharomyces cerevisiae
Peptides
DNA
Switches
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Broach, James ; Atkins, John F. ; McGill, Carolyn ; Chow, Louise. / Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2μ circle. In: Cell. 1979 ; Vol. 16, No. 4. pp. 827-839.
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Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2μ circle. / Broach, James; Atkins, John F.; McGill, Carolyn; Chow, Louise.

In: Cell, Vol. 16, No. 4, 01.01.1979, p. 827-839.

Research output: Contribution to journalArticle

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N2 - We have identified two major and approximately ten minor poly(A)-containing RNA species in S. cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2μ circle. The two major species, which are 1325 and 1275 bases in length, are transcribed from the two unique halves of the plasmid and extend into the inverted repeat sequences which separate the unique regions. The map positions of the minor transcripts, which range in length from 350 to 2600 bases, indicate that except for a small region of the genome in which no transcription is observed, both strands of the entire 2μ circle genome are transcribed. We also present evidence demonstrating that RNA transcribed from 2μ circular DNA is used to program the synthesis of specific proteins in yeast: that is, yeast RNA complementary to 2μ circle DNA can be translated in vitro to produce specific polypeptides of substantial size. Finally, the pattern of transcription of 2μ circle suggests the possibility that messenger RNA species are derived by cleavage of larger transcripts, and in addition, that the intramolecular recombination of 2μ circle which occurs in yeast functions as a genetic switch to allow separate expression of two sets of genes on the 2μ circle genome.

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