Identification and use of fluorescent dyes for plant cell wall imaging using high-throughput screening

Charles T. Anderson, Andrew Carroll

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Plant cell walls define cell shape during development and are composed of interlaced carbohydrate and protein networks. Fluorescent dyes have long been used to label plant cell walls, enabling optical microscopy-based interrogation of cell wall structure and composition. However, the specific cell wall components to which these dyes bind are often poorly defined. The availability of fluorescent compound libraries provides the potential to screen for and identify new fluorescent compounds that interact with specific plant cell wall components, enabling the study of cell wall architecture in intact, living tissues. Here, we describe a technique for screening fluorescent compound libraries for enhanced fluorescence upon interaction with plant cell walls, a secondary screening method to identify which cell wall components interact with a given dye, and a protocol for staining and observing Arabidopsis seedlings using a fluorescent cell wall-labeling dye. These methods have the potential to be applied to screening for differences in cell wall structure and composition among genetically diverse plant varieties or species.

Original languageEnglish (US)
Title of host publicationPlant Chemical Genomics: Methods and Protocols
PublisherHumana Press Inc.
Pages103-109
Number of pages7
Volume1056
ISBN (Print)9781627035910
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1056
ISSN (Print)10643745

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Anderson, C. T., & Carroll, A. (2014). Identification and use of fluorescent dyes for plant cell wall imaging using high-throughput screening. In Plant Chemical Genomics: Methods and Protocols (Vol. 1056, pp. 103-109). (Methods in Molecular Biology; Vol. 1056). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-592-7_10