Identification, cloning, and sequencing of DNA essential for encapsulation of Streptococcus pneumoniae

David A. Watson, Vivek Kapur, Daniel M. Musher, James W. Jacobson, James M. Musser

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This paper reports the cloning and sequencing of a region of DNA from Streptococcus pneumoniae serotype 3 surrounding transposon Tn 916, insertion of which was previously shown to result in lack of expression of the extracellular capsule. Sequence analysis revealed that the transposon inserted into a consensus insertion site 71 bp from the 5′ end of the cloned fragment. Within the clone, 3′ downstream regions from two different pneumococcal lytA genes were identified, as well as a putative 194 AA open reading frame (ORF1). Moreover, two copies of the repeat element BOX, oriented in opposite directions, were located immediately 3′ of orf1. Within the region bounded by the first pair of internal sequencing primers, analysis revealed that the fragment amplified by PCR was always of the same size. Moreover, Southern blotting showed that for all serotypes examined to date, homology exists with the cloned fragment. These results indicate that this region of the chromosome is highly conserved and, taken together with other independently derived data, suggest that interruptions or deletions within this DNA lead to unencapsulation.

Original languageEnglish (US)
Pages (from-to)251-259
Number of pages9
JournalCurrent Microbiology
Volume31
Issue number4
DOIs
StatePublished - Oct 1 1995

Fingerprint

Streptococcus pneumoniae
DNA Sequence Analysis
Organism Cloning
DNA
Southern Blotting
Open Reading Frames
Capsules
Sequence Analysis
Clone Cells
Chromosomes
Polymerase Chain Reaction
Genes
Serogroup
Direction compound

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Watson, David A. ; Kapur, Vivek ; Musher, Daniel M. ; Jacobson, James W. ; Musser, James M. / Identification, cloning, and sequencing of DNA essential for encapsulation of Streptococcus pneumoniae. In: Current Microbiology. 1995 ; Vol. 31, No. 4. pp. 251-259.
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Identification, cloning, and sequencing of DNA essential for encapsulation of Streptococcus pneumoniae. / Watson, David A.; Kapur, Vivek; Musher, Daniel M.; Jacobson, James W.; Musser, James M.

In: Current Microbiology, Vol. 31, No. 4, 01.10.1995, p. 251-259.

Research output: Contribution to journalArticle

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