Identification of a cellular receptor for subgroup E avian leukosis virus

Heather B. Adkins, Jürgen Brojatsch, John Naughton, Melissa Rolls, Jean M. Pesola, John A.T. Young

Research output: Contribution to journalArticle

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Abstract

Genetic studies in chickens and receptor interference experiments have indicated that avian leukosis virus (ALV)-E may utilize a cellular receptor related to the receptor for ALV-B and ALV-D. Recently, we cloned CAR1, a tumor necrosis factor receptor (TNFR)-related protein, that serves as a cellular receptor for ALV-B and ALV-D. To determine whether the cellular receptor for ALV-E is a CAR1-like protein, a cDNA library was made from turkey embryo fibroblasts (TEFs), which are susceptible to ALV-E infection, but not to infection by ALV-B and ALV-D. The cDNA library was screened with a radioactively labeled CAR1 cDNA probe, and clones that hybridized with the probe were isolated. A 2.3-kb cDNA clone was identified that conferred susceptibility to ALV-E infection, but not to ALV-B infection, when expressed in transfected human 293 cells. The functional cDNA clone is predicted to encode a 368 amino acid protein with significant amino acid similarity to CAR1. Like CAR1, the TEF protein is predicted to have two extracellular TNFR- like cysteine-rich domains and a putative death domain similar to those of TNFR I and Fas. Flow cytometric analysis and immunoprecipitation experiments demonstrated specific binding between the TEF CAR1-related protein and an immunoadhesin composed of the surface (SU) envelope protein of subgroup E (RAV-0) virus fused to the constant region of a rabbit immunoglobulin. These two activities of the TEF CAR1-related protein, specific binding to ALV-E SU and permitting entry only of ALV-E, have unambiguously identified this protein as a cellular receptor specific for subgroup E ALV.

Original languageEnglish (US)
Pages (from-to)11617-11622
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number21
DOIs
StatePublished - Oct 14 1997

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Avian Leukosis Virus
Tumor Necrosis Factor Receptors
Virus Diseases
Embryonic Structures
Fibroblasts
Proteins
Complementary DNA
Clone Cells
Gene Library
Amino Acids

All Science Journal Classification (ASJC) codes

  • General

Cite this

Adkins, Heather B. ; Brojatsch, Jürgen ; Naughton, John ; Rolls, Melissa ; Pesola, Jean M. ; Young, John A.T. / Identification of a cellular receptor for subgroup E avian leukosis virus. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 21. pp. 11617-11622.
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Identification of a cellular receptor for subgroup E avian leukosis virus. / Adkins, Heather B.; Brojatsch, Jürgen; Naughton, John; Rolls, Melissa; Pesola, Jean M.; Young, John A.T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 21, 14.10.1997, p. 11617-11622.

Research output: Contribution to journalArticle

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AU - Adkins, Heather B.

AU - Brojatsch, Jürgen

AU - Naughton, John

AU - Rolls, Melissa

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AU - Young, John A.T.

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