Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy - 2009-2011

Ettore Amato, Virginia Filipello, Maria Gori, Sara Lomonaco, Marina Nadia Losio, Antonio Parisi, Pol Huedo, Stephen John Knabel, Mirella Pontello

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Abstract

Background: Molecular subtyping and enhanced surveillance in Lombardy region identified a cluster of possibly related listeriosis cases from 2006 to 2010. This cluster grouped 31 isolates that belonged to serotype 1/2a and Sequence Type 38 (ST38) as defined by Multilocus Sequence Typing (MLST). Methods: Our study expanded the previous investigation to include cases from 2011 to 2014 and used Multi-Virulence-Locus Sequence Typing (MVLST) on all ST38 isolates to better understand their epidemiology and possibly identify a common source outbreak. Results: Out of 306L. monocytogenes clinical isolates collected, 43 (14.1%) belonged to ST38 with cases occurring in nine out of twelve Lombardy provinces. The ST38 isolates were split by MVLST into two Virulence Types (VTs): VT80 (n=12) and VT104 (n=31). VT104 cases were concentrated between 2009 and 2011 in two provinces, Bergamo and Milan. An epidemiologic investigation was performed and in one case, a matching VT104 isolate was retrieved from a soft cheese sample from a patient's refrigerator. Conclusions: Our findings revealed a major listeriosis outbreak in Northern Italy linked to soft cheese in 2009-2011, which went undetected by local health authorities. Our study shows that integrating subtyping methods with conventional epidemiology can help identify the source of L. monocytogenes outbreak clones.

Original languageEnglish (US)
Article number342
JournalBMC Infectious Diseases
Volume17
Issue number1
DOIs
StatePublished - May 12 2017

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Cheese
Listeria monocytogenes
Italy
Disease Outbreaks
Virulence
Listeriosis
Clone Cells
Epidemiology
Multilocus Sequence Typing
Health

All Science Journal Classification (ASJC) codes

  • Infectious Diseases

Cite this

Amato, Ettore ; Filipello, Virginia ; Gori, Maria ; Lomonaco, Sara ; Losio, Marina Nadia ; Parisi, Antonio ; Huedo, Pol ; Knabel, Stephen John ; Pontello, Mirella. / Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy - 2009-2011. In: BMC Infectious Diseases. 2017 ; Vol. 17, No. 1.
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abstract = "Background: Molecular subtyping and enhanced surveillance in Lombardy region identified a cluster of possibly related listeriosis cases from 2006 to 2010. This cluster grouped 31 isolates that belonged to serotype 1/2a and Sequence Type 38 (ST38) as defined by Multilocus Sequence Typing (MLST). Methods: Our study expanded the previous investigation to include cases from 2011 to 2014 and used Multi-Virulence-Locus Sequence Typing (MVLST) on all ST38 isolates to better understand their epidemiology and possibly identify a common source outbreak. Results: Out of 306L. monocytogenes clinical isolates collected, 43 (14.1{\%}) belonged to ST38 with cases occurring in nine out of twelve Lombardy provinces. The ST38 isolates were split by MVLST into two Virulence Types (VTs): VT80 (n=12) and VT104 (n=31). VT104 cases were concentrated between 2009 and 2011 in two provinces, Bergamo and Milan. An epidemiologic investigation was performed and in one case, a matching VT104 isolate was retrieved from a soft cheese sample from a patient's refrigerator. Conclusions: Our findings revealed a major listeriosis outbreak in Northern Italy linked to soft cheese in 2009-2011, which went undetected by local health authorities. Our study shows that integrating subtyping methods with conventional epidemiology can help identify the source of L. monocytogenes outbreak clones.",
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Amato, E, Filipello, V, Gori, M, Lomonaco, S, Losio, MN, Parisi, A, Huedo, P, Knabel, SJ & Pontello, M 2017, 'Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy - 2009-2011', BMC Infectious Diseases, vol. 17, no. 1, 342. https://doi.org/10.1186/s12879-017-2441-6

Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy - 2009-2011. / Amato, Ettore; Filipello, Virginia; Gori, Maria; Lomonaco, Sara; Losio, Marina Nadia; Parisi, Antonio; Huedo, Pol; Knabel, Stephen John; Pontello, Mirella.

In: BMC Infectious Diseases, Vol. 17, No. 1, 342, 12.05.2017.

Research output: Contribution to journalArticle

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AU - Amato, Ettore

AU - Filipello, Virginia

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AU - Lomonaco, Sara

AU - Losio, Marina Nadia

AU - Parisi, Antonio

AU - Huedo, Pol

AU - Knabel, Stephen John

AU - Pontello, Mirella

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AB - Background: Molecular subtyping and enhanced surveillance in Lombardy region identified a cluster of possibly related listeriosis cases from 2006 to 2010. This cluster grouped 31 isolates that belonged to serotype 1/2a and Sequence Type 38 (ST38) as defined by Multilocus Sequence Typing (MLST). Methods: Our study expanded the previous investigation to include cases from 2011 to 2014 and used Multi-Virulence-Locus Sequence Typing (MVLST) on all ST38 isolates to better understand their epidemiology and possibly identify a common source outbreak. Results: Out of 306L. monocytogenes clinical isolates collected, 43 (14.1%) belonged to ST38 with cases occurring in nine out of twelve Lombardy provinces. The ST38 isolates were split by MVLST into two Virulence Types (VTs): VT80 (n=12) and VT104 (n=31). VT104 cases were concentrated between 2009 and 2011 in two provinces, Bergamo and Milan. An epidemiologic investigation was performed and in one case, a matching VT104 isolate was retrieved from a soft cheese sample from a patient's refrigerator. Conclusions: Our findings revealed a major listeriosis outbreak in Northern Italy linked to soft cheese in 2009-2011, which went undetected by local health authorities. Our study shows that integrating subtyping methods with conventional epidemiology can help identify the source of L. monocytogenes outbreak clones.

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