Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections

Le Van Tan, H. Rogier van Doorn, Ho Dang Trung Nghia, Tran Thi Hong Chau, Le Thi Phuong Tu, Michel de Vries, Marta Canuti, Martin Deijs, Maarten F. Jebbink, Stephen Baker, Juliet E. Bryant, Nguyen Thi Tham, Nguyen Thi Thuy Chinh BKrong, Maciej F. Boni, Tran Quoc Loi, Le Thi Phuong, Joost T.P. Verhoeven, Martin Crusat, Rienk E. Jeeninga, Constance SchultszNguyen Van Vinh Chau, Tran Tinh Hien, Lia van der Hoek, Jeremy Farrar, Menno D. de Jong

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal fluid (CSF) specimens of two Vietnamese patients with CNS infections of unknown etiology. CyCV-VN was subsequently detected in 4% of 642 CSF specimens from Vietnamese patients with suspected CNS infections and none of 122 CSFs from patients with noninfectious neurological disorders. Detection rates were similar in patients with CNS infections of unknown etiology and those in whom other pathogens were detected. A similar detection rate in feces from healthy children suggested food-borne or orofecal transmission routes, while high detection rates in feces from pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further research is needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus. IMPORTANCE Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology frequently remains unknown, which hampers development of therapeutic or preventive strategies. Hence, identification of novel pathogens is essential and is facilitated by current next-generation sequencing-based methods. Using such technology, we identified and characterized the full genome of a novel cyclovirus in cerebrospinal fluid (CSF) specimens from two Vietnamese patients with CNS infections of unknown etiology, which was subsequently detected in none of 122 CSF specimens from patients with noninfectious neurological disorders but 4% of 642 CSF specimens from Vietnamese patients with suspected or confirmed CNS infections. Similar detection rates in feces from healthy children suggested food-borne or orofecal transmission routes, while frequent detection in feces from Vietnamese pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further studies are needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus.

Original languageEnglish (US)
JournalmBio
Volume4
Issue number3
DOIs
StatePublished - Jan 1 2013

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Central Nervous System Infections
Cerebrospinal Fluid
Feces
Vietnam
Zoonoses
Poultry
Nervous System Diseases
Virulence
Epidemiology
Swine
Genome
Viruses
Morbidity
Food
Mortality
Technology
Research

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Virology

Cite this

Tan, L. V., van Doorn, H. R., Nghia, H. D. T., Chau, T. T. H., Tu, L. T. P., de Vries, M., ... de Jong, M. D. (2013). Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections. mBio, 4(3). https://doi.org/10.1128/mBio.00231-13
Tan, Le Van ; van Doorn, H. Rogier ; Nghia, Ho Dang Trung ; Chau, Tran Thi Hong ; Tu, Le Thi Phuong ; de Vries, Michel ; Canuti, Marta ; Deijs, Martin ; Jebbink, Maarten F. ; Baker, Stephen ; Bryant, Juliet E. ; Tham, Nguyen Thi ; BKrong, Nguyen Thi Thuy Chinh ; Boni, Maciej F. ; Loi, Tran Quoc ; Phuong, Le Thi ; Verhoeven, Joost T.P. ; Crusat, Martin ; Jeeninga, Rienk E. ; Schultsz, Constance ; Chau, Nguyen Van Vinh ; Hien, Tran Tinh ; van der Hoek, Lia ; Farrar, Jeremy ; de Jong, Menno D. / Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections. In: mBio. 2013 ; Vol. 4, No. 3.
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Tan, LV, van Doorn, HR, Nghia, HDT, Chau, TTH, Tu, LTP, de Vries, M, Canuti, M, Deijs, M, Jebbink, MF, Baker, S, Bryant, JE, Tham, NT, BKrong, NTTC, Boni, MF, Loi, TQ, Phuong, LT, Verhoeven, JTP, Crusat, M, Jeeninga, RE, Schultsz, C, Chau, NVV, Hien, TT, van der Hoek, L, Farrar, J & de Jong, MD 2013, 'Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections', mBio, vol. 4, no. 3. https://doi.org/10.1128/mBio.00231-13

Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections. / Tan, Le Van; van Doorn, H. Rogier; Nghia, Ho Dang Trung; Chau, Tran Thi Hong; Tu, Le Thi Phuong; de Vries, Michel; Canuti, Marta; Deijs, Martin; Jebbink, Maarten F.; Baker, Stephen; Bryant, Juliet E.; Tham, Nguyen Thi; BKrong, Nguyen Thi Thuy Chinh; Boni, Maciej F.; Loi, Tran Quoc; Phuong, Le Thi; Verhoeven, Joost T.P.; Crusat, Martin; Jeeninga, Rienk E.; Schultsz, Constance; Chau, Nguyen Van Vinh; Hien, Tran Tinh; van der Hoek, Lia; Farrar, Jeremy; de Jong, Menno D.

In: mBio, Vol. 4, No. 3, 01.01.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections

AU - Tan, Le Van

AU - van Doorn, H. Rogier

AU - Nghia, Ho Dang Trung

AU - Chau, Tran Thi Hong

AU - Tu, Le Thi Phuong

AU - de Vries, Michel

AU - Canuti, Marta

AU - Deijs, Martin

AU - Jebbink, Maarten F.

AU - Baker, Stephen

AU - Bryant, Juliet E.

AU - Tham, Nguyen Thi

AU - BKrong, Nguyen Thi Thuy Chinh

AU - Boni, Maciej F.

AU - Loi, Tran Quoc

AU - Phuong, Le Thi

AU - Verhoeven, Joost T.P.

AU - Crusat, Martin

AU - Jeeninga, Rienk E.

AU - Schultsz, Constance

AU - Chau, Nguyen Van Vinh

AU - Hien, Tran Tinh

AU - van der Hoek, Lia

AU - Farrar, Jeremy

AU - de Jong, Menno D.

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal fluid (CSF) specimens of two Vietnamese patients with CNS infections of unknown etiology. CyCV-VN was subsequently detected in 4% of 642 CSF specimens from Vietnamese patients with suspected CNS infections and none of 122 CSFs from patients with noninfectious neurological disorders. Detection rates were similar in patients with CNS infections of unknown etiology and those in whom other pathogens were detected. A similar detection rate in feces from healthy children suggested food-borne or orofecal transmission routes, while high detection rates in feces from pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further research is needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus. IMPORTANCE Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology frequently remains unknown, which hampers development of therapeutic or preventive strategies. Hence, identification of novel pathogens is essential and is facilitated by current next-generation sequencing-based methods. Using such technology, we identified and characterized the full genome of a novel cyclovirus in cerebrospinal fluid (CSF) specimens from two Vietnamese patients with CNS infections of unknown etiology, which was subsequently detected in none of 122 CSF specimens from patients with noninfectious neurological disorders but 4% of 642 CSF specimens from Vietnamese patients with suspected or confirmed CNS infections. Similar detection rates in feces from healthy children suggested food-borne or orofecal transmission routes, while frequent detection in feces from Vietnamese pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further studies are needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus.

AB - Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal fluid (CSF) specimens of two Vietnamese patients with CNS infections of unknown etiology. CyCV-VN was subsequently detected in 4% of 642 CSF specimens from Vietnamese patients with suspected CNS infections and none of 122 CSFs from patients with noninfectious neurological disorders. Detection rates were similar in patients with CNS infections of unknown etiology and those in whom other pathogens were detected. A similar detection rate in feces from healthy children suggested food-borne or orofecal transmission routes, while high detection rates in feces from pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further research is needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus. IMPORTANCE Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology frequently remains unknown, which hampers development of therapeutic or preventive strategies. Hence, identification of novel pathogens is essential and is facilitated by current next-generation sequencing-based methods. Using such technology, we identified and characterized the full genome of a novel cyclovirus in cerebrospinal fluid (CSF) specimens from two Vietnamese patients with CNS infections of unknown etiology, which was subsequently detected in none of 122 CSF specimens from patients with noninfectious neurological disorders but 4% of 642 CSF specimens from Vietnamese patients with suspected or confirmed CNS infections. Similar detection rates in feces from healthy children suggested food-borne or orofecal transmission routes, while frequent detection in feces from Vietnamese pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further studies are needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus.

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