Identification of a novel negative role of flagellin in regulating IL-10 production

Ildefonso Vicente-Suarez, Yoshinori Takahashi, Fengdong Cheng, Pedro Horna, Hong W. Wang, Hong Gang Wang, Eduardo M. Sotomayor

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Toll-like receptors (TLR) expressed by cells of the immune system play a central role in the generation of immune responses against pathogens. Following TLR ligation, both pro-inflammatory and anti-inflammatory mediators are produced in order to elicit an immune response that controls the microbial infection while limiting tissue damage. Among these mediators, the pro-inflammatory cytokine IL-12 and the anti-inflammatory cytokine IL-10 are known to play major roles. Here, we show that in vitro or in vivo stimulation with flagellin, the TLR5 ligand, does not result in IL-10 production. Furthermore, flagellin inhibits IL-10 production by other specific TLR ligands at the protein and mRNA levels while increasing IL-12p70 production. Several studies have linked the activation of extracellular signal-regulated kinases with IL-10 induction by TLR. We have observed that LPS-induced extracellular signal-regulated kinase activation was significantly decreased in flagellin-treated macrophages, suggesting that this pathway might play a role in the inhibition of IL-10 production observed in flagellin-treated macrophages. Flagellin-mediated IL-10 inhibition was not observed in cells that do not express TLR5, supporting that this effect is indeed TLR5-dependent. This study provides a new insight into the role of flagellin recognition by TLR5 in shaping the immune response elicited by flagellated microorganisms.

Original languageEnglish (US)
Pages (from-to)3164-3175
Number of pages12
JournalEuropean Journal of Immunology
Volume37
Issue number11
DOIs
StatePublished - Nov 1 2007

Fingerprint

Flagellin
Interleukin-10
Toll-Like Receptors
Extracellular Signal-Regulated MAP Kinases
Anti-Inflammatory Agents
Macrophages
Cytokines
Ligands
Interleukin-12
Infection Control
Ligation
Immune System
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Vicente-Suarez, Ildefonso ; Takahashi, Yoshinori ; Cheng, Fengdong ; Horna, Pedro ; Wang, Hong W. ; Wang, Hong Gang ; Sotomayor, Eduardo M. / Identification of a novel negative role of flagellin in regulating IL-10 production. In: European Journal of Immunology. 2007 ; Vol. 37, No. 11. pp. 3164-3175.
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Identification of a novel negative role of flagellin in regulating IL-10 production. / Vicente-Suarez, Ildefonso; Takahashi, Yoshinori; Cheng, Fengdong; Horna, Pedro; Wang, Hong W.; Wang, Hong Gang; Sotomayor, Eduardo M.

In: European Journal of Immunology, Vol. 37, No. 11, 01.11.2007, p. 3164-3175.

Research output: Contribution to journalArticle

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