Identification of a potentially important antigen of pasteurella haemolytica

S. K. Weldon, D. A. Mosier, K. R. Simons, R. C. Craven, A. W. Confer

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To identify antigens which may be important for stimulating immunity to pneumonic pasteurellosis, a bovine antiserum to whole P. haemolytica was used to screen a recombinant lambda gt11/P. haemolytica expression library. One of the recombinant bacteriophage clones identified with the bovine antiserum, SW20C, expressed a fusion protein which was also recognized by rabbit antiserum to partially purified P. haemolytica culture supernatant and was found to be immunogenic in guinea pigs. The guinea pig antibody recognized a 100 kDa protein in P. haemolytica cell lysates. Sequence analysis of the cloned DNA from SW20C identified a fragment of 1443 bp with a small open reading frame that was contiguous with the lacZ sequence. The 153 bp P. haemolytica-specific open reading frame encoded a polypeptide of approximately 6kDa. Homology searches of Genbank and the EMBL data bases revealed no homology of this open reading frame with any other bacterial sequences including P. haemolytica leukotoxin and Ssa1. Evaluation of sera from calves that were scored either susceptible or resistant to experimental pneumonic pasteurellosis demonstrated a significant (P < 0.001) correlation between the intensity of the antibody response to the SW20C antigen and resistance to disease.

Original languageEnglish (US)
Pages (from-to)283-291
Number of pages9
JournalVeterinary Microbiology
Volume40
Issue number3-4
DOIs
StatePublished - Jun 1994

Fingerprint

Mannheimia haemolytica
antigens
Antigens
Pneumonic Pasteurellosis
Open Reading Frames
pneumonic pasteurellosis
antiserum
open reading frames
Immune Sera
guinea pigs
Guinea Pigs
leukotoxins
antibodies
Disease Resistance
cattle
Nucleic Acid Databases
blood serum
DNA Sequence Analysis
bacteriophages
Bacteriophages

All Science Journal Classification (ASJC) codes

  • Microbiology
  • veterinary(all)

Cite this

Weldon, S. K. ; Mosier, D. A. ; Simons, K. R. ; Craven, R. C. ; Confer, A. W. / Identification of a potentially important antigen of pasteurella haemolytica. In: Veterinary Microbiology. 1994 ; Vol. 40, No. 3-4. pp. 283-291.
@article{844bc425d8ca4748aff6e3c3f6ced058,
title = "Identification of a potentially important antigen of pasteurella haemolytica",
abstract = "To identify antigens which may be important for stimulating immunity to pneumonic pasteurellosis, a bovine antiserum to whole P. haemolytica was used to screen a recombinant lambda gt11/P. haemolytica expression library. One of the recombinant bacteriophage clones identified with the bovine antiserum, SW20C, expressed a fusion protein which was also recognized by rabbit antiserum to partially purified P. haemolytica culture supernatant and was found to be immunogenic in guinea pigs. The guinea pig antibody recognized a 100 kDa protein in P. haemolytica cell lysates. Sequence analysis of the cloned DNA from SW20C identified a fragment of 1443 bp with a small open reading frame that was contiguous with the lacZ sequence. The 153 bp P. haemolytica-specific open reading frame encoded a polypeptide of approximately 6kDa. Homology searches of Genbank and the EMBL data bases revealed no homology of this open reading frame with any other bacterial sequences including P. haemolytica leukotoxin and Ssa1. Evaluation of sera from calves that were scored either susceptible or resistant to experimental pneumonic pasteurellosis demonstrated a significant (P < 0.001) correlation between the intensity of the antibody response to the SW20C antigen and resistance to disease.",
author = "Weldon, {S. K.} and Mosier, {D. A.} and Simons, {K. R.} and Craven, {R. C.} and Confer, {A. W.}",
year = "1994",
month = "6",
doi = "10.1016/0378-1135(94)90117-1",
language = "English (US)",
volume = "40",
pages = "283--291",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",
number = "3-4",

}

Identification of a potentially important antigen of pasteurella haemolytica. / Weldon, S. K.; Mosier, D. A.; Simons, K. R.; Craven, R. C.; Confer, A. W.

In: Veterinary Microbiology, Vol. 40, No. 3-4, 06.1994, p. 283-291.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of a potentially important antigen of pasteurella haemolytica

AU - Weldon, S. K.

AU - Mosier, D. A.

AU - Simons, K. R.

AU - Craven, R. C.

AU - Confer, A. W.

PY - 1994/6

Y1 - 1994/6

N2 - To identify antigens which may be important for stimulating immunity to pneumonic pasteurellosis, a bovine antiserum to whole P. haemolytica was used to screen a recombinant lambda gt11/P. haemolytica expression library. One of the recombinant bacteriophage clones identified with the bovine antiserum, SW20C, expressed a fusion protein which was also recognized by rabbit antiserum to partially purified P. haemolytica culture supernatant and was found to be immunogenic in guinea pigs. The guinea pig antibody recognized a 100 kDa protein in P. haemolytica cell lysates. Sequence analysis of the cloned DNA from SW20C identified a fragment of 1443 bp with a small open reading frame that was contiguous with the lacZ sequence. The 153 bp P. haemolytica-specific open reading frame encoded a polypeptide of approximately 6kDa. Homology searches of Genbank and the EMBL data bases revealed no homology of this open reading frame with any other bacterial sequences including P. haemolytica leukotoxin and Ssa1. Evaluation of sera from calves that were scored either susceptible or resistant to experimental pneumonic pasteurellosis demonstrated a significant (P < 0.001) correlation between the intensity of the antibody response to the SW20C antigen and resistance to disease.

AB - To identify antigens which may be important for stimulating immunity to pneumonic pasteurellosis, a bovine antiserum to whole P. haemolytica was used to screen a recombinant lambda gt11/P. haemolytica expression library. One of the recombinant bacteriophage clones identified with the bovine antiserum, SW20C, expressed a fusion protein which was also recognized by rabbit antiserum to partially purified P. haemolytica culture supernatant and was found to be immunogenic in guinea pigs. The guinea pig antibody recognized a 100 kDa protein in P. haemolytica cell lysates. Sequence analysis of the cloned DNA from SW20C identified a fragment of 1443 bp with a small open reading frame that was contiguous with the lacZ sequence. The 153 bp P. haemolytica-specific open reading frame encoded a polypeptide of approximately 6kDa. Homology searches of Genbank and the EMBL data bases revealed no homology of this open reading frame with any other bacterial sequences including P. haemolytica leukotoxin and Ssa1. Evaluation of sera from calves that were scored either susceptible or resistant to experimental pneumonic pasteurellosis demonstrated a significant (P < 0.001) correlation between the intensity of the antibody response to the SW20C antigen and resistance to disease.

UR - http://www.scopus.com/inward/record.url?scp=0027984987&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027984987&partnerID=8YFLogxK

U2 - 10.1016/0378-1135(94)90117-1

DO - 10.1016/0378-1135(94)90117-1

M3 - Article

C2 - 7941293

AN - SCOPUS:0027984987

VL - 40

SP - 283

EP - 291

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -