Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs

P. K. Ghosh, P. Lebowitz, R. J. Frisque, Y. Gluzman

Research output: Contribution to journalArticle

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Abstract

The 5'termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template. The 5'termini of early mRNAs produced by five origin-defective mutants containing deletions downstream from the A-T-T-T-A-T sequence and one viable mutant dl892 with a deletion starting 15 nucleotides upstream from this sequence were determined by a method involving synthesis, separation and, determination of the sequences of DNAs complementary to 5' termini. Mutant dl892 produced early mRNAs with the same principal 5' termini as wild-type virus; the origin-defective mutants produced mRNAs with principal 5'termini shifted downstream by a distance equivalent to the length of the deleted DNA segment. These data suggest that a DNA sequence of 29 nucleotides, which includes the A-T-T-T-A-T sequence, contains a component(s) of a promoter for early transcription. This component functions in positioning the 5' ends of the principal early mRNAs 21-25 nucleotides downstream from the A-T-T-T-A-T sequence and acts independently of these downstream sequences.

Original languageEnglish (US)
Pages (from-to)100-104
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number1 II
StatePublished - 1981

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Simian virus 40
Messenger RNA
Nucleotides
DNA Sequence Analysis
Complementary DNA
Viruses
DNA

All Science Journal Classification (ASJC) codes

  • General
  • Genetics

Cite this

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title = "Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs",
abstract = "The 5'termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template. The 5'termini of early mRNAs produced by five origin-defective mutants containing deletions downstream from the A-T-T-T-A-T sequence and one viable mutant dl892 with a deletion starting 15 nucleotides upstream from this sequence were determined by a method involving synthesis, separation and, determination of the sequences of DNAs complementary to 5' termini. Mutant dl892 produced early mRNAs with the same principal 5' termini as wild-type virus; the origin-defective mutants produced mRNAs with principal 5'termini shifted downstream by a distance equivalent to the length of the deleted DNA segment. These data suggest that a DNA sequence of 29 nucleotides, which includes the A-T-T-T-A-T sequence, contains a component(s) of a promoter for early transcription. This component functions in positioning the 5' ends of the principal early mRNAs 21-25 nucleotides downstream from the A-T-T-T-A-T sequence and acts independently of these downstream sequences.",
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Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs. / Ghosh, P. K.; Lebowitz, P.; Frisque, R. J.; Gluzman, Y.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 1 II, 1981, p. 100-104.

Research output: Contribution to journalArticle

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AU - Ghosh, P. K.

AU - Lebowitz, P.

AU - Frisque, R. J.

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AB - The 5'termini of the principal early mRNAs produced in cells transformed by wild-type simian virus 40 lie 21-25 nucleotides downstream from an A-T-T-T-A-T sequence on the DNA template. The 5'termini of early mRNAs produced by five origin-defective mutants containing deletions downstream from the A-T-T-T-A-T sequence and one viable mutant dl892 with a deletion starting 15 nucleotides upstream from this sequence were determined by a method involving synthesis, separation and, determination of the sequences of DNAs complementary to 5' termini. Mutant dl892 produced early mRNAs with the same principal 5' termini as wild-type virus; the origin-defective mutants produced mRNAs with principal 5'termini shifted downstream by a distance equivalent to the length of the deleted DNA segment. These data suggest that a DNA sequence of 29 nucleotides, which includes the A-T-T-T-A-T sequence, contains a component(s) of a promoter for early transcription. This component functions in positioning the 5' ends of the principal early mRNAs 21-25 nucleotides downstream from the A-T-T-T-A-T sequence and acts independently of these downstream sequences.

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