Identification of a second Asian soybean rust resistance gene in Hyuuga soybean

Mandy D. Kendrick, Donna K. Harris, Bo Keun Ha, David L. Hyten, Perry B. Cregan, Reid D. Frederick, H. Roger Boerma, Kerry F. Pedley

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Asian soybean rust (ASR) is an economically significant disease caused by the fungus Phakopsora pachyrhizi. The soybean genes Rpp3 and Rpp?(Hyuuga) confer resistance to specific isolates of the pathogen. Both genes map to chromosome 6 (Gm06) (linkage group [LG] C2). We recently identified 12 additional soybean accessions that harbor ASR resistance mapping to Gm06, within 5 centimorgans of Rpp3 and Rpp?(Hyuuga). To further characterize genotypes with resistance on Gm06, we used a set of eight P. pachyrhizi isolates collected from geographically diverse areas to inoculate plants and evaluate them for differential phenotypic responses. Three isolates elicited different responses from soybean accessions PI 462312 (Ankur) (Rpp3) and PI 506764 (Hyuuga) (Rpp?[Hyuuga]). In all, 11 of the new accessions yielded responses identical to either PI 462312 or Hyuuga and 1 of the new accessions, PI 417089B (Kuro daizu), differed from all others. Additional screening of Hyuuga-derived recombinant inbred lines indicated that Hyuuga carries two resistance genes, one at the Rpp3 locus on Gm06 and a second, unlinked ASR resistance gene mapping to Gm03 (LG-N) near Rpp5. These findings reveal a natural case of gene pyramiding for ASR resistance in Hyuuga and underscore the importance of utilizing multiple isolates of P. pachyrhizi when screening for ASR resistance.

Original languageEnglish (US)
Pages (from-to)535-543
Number of pages9
JournalPHYTOPATHOLOGY
Volume101
Issue number5
DOIs
StatePublished - May 1 2011

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soybean rust
Phakopsora pachyrhizi
soybeans
linkage groups
genes
screening
inbred lines
chromosome mapping
chromosomes
loci
fungi
pathogens
genotype

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Kendrick, M. D., Harris, D. K., Ha, B. K., Hyten, D. L., Cregan, P. B., Frederick, R. D., ... Pedley, K. F. (2011). Identification of a second Asian soybean rust resistance gene in Hyuuga soybean. PHYTOPATHOLOGY, 101(5), 535-543. https://doi.org/10.1094/PHYTO-09-10-0257
Kendrick, Mandy D. ; Harris, Donna K. ; Ha, Bo Keun ; Hyten, David L. ; Cregan, Perry B. ; Frederick, Reid D. ; Boerma, H. Roger ; Pedley, Kerry F. / Identification of a second Asian soybean rust resistance gene in Hyuuga soybean. In: PHYTOPATHOLOGY. 2011 ; Vol. 101, No. 5. pp. 535-543.
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Kendrick, MD, Harris, DK, Ha, BK, Hyten, DL, Cregan, PB, Frederick, RD, Boerma, HR & Pedley, KF 2011, 'Identification of a second Asian soybean rust resistance gene in Hyuuga soybean', PHYTOPATHOLOGY, vol. 101, no. 5, pp. 535-543. https://doi.org/10.1094/PHYTO-09-10-0257

Identification of a second Asian soybean rust resistance gene in Hyuuga soybean. / Kendrick, Mandy D.; Harris, Donna K.; Ha, Bo Keun; Hyten, David L.; Cregan, Perry B.; Frederick, Reid D.; Boerma, H. Roger; Pedley, Kerry F.

In: PHYTOPATHOLOGY, Vol. 101, No. 5, 01.05.2011, p. 535-543.

Research output: Contribution to journalArticle

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Kendrick MD, Harris DK, Ha BK, Hyten DL, Cregan PB, Frederick RD et al. Identification of a second Asian soybean rust resistance gene in Hyuuga soybean. PHYTOPATHOLOGY. 2011 May 1;101(5):535-543. https://doi.org/10.1094/PHYTO-09-10-0257