Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties

Peter M. Thompson, Caitlin E. Tolbert, Kai Shen, Pradeep Kota, Sean M. Palmer, Karen M. Plevock, Albina Orlova, Vitold E. Galkin, Keith Burridge, Edward H. Egelman, Nikolay Dokholyan, Richard Superfine, Sharon L. Campbell

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Summary Vinculin, a cytoskeletal scaffold protein essential for embryogenesis and cardiovascular function, localizes to focal adhesions and adherens junctions, connecting cell surface receptors to the actin cytoskeleton. While vinculin interacts with many adhesion proteins, its interaction with filamentous actin regulates cell morphology, motility, and mechanotransduction. Disruption of this interaction lowers cell traction forces and enhances actin flow rates. Although a model for the vinculin:actin complex exists, we recently identified actin-binding deficient mutants of vinculin outside sites predicted to bind actin and developed an alternative model to better define this actin-binding surface, using negative-stain electron microscopy (EM), discrete molecular dynamics, and mutagenesis. Actin-binding deficient vinculin variants expressed in vinculin knockout fibroblasts fail to rescue cell-spreading defects and reduce cellular response to external force. These findings highlight the importance of this actin-binding surface and provide the molecular basis for elucidating additional roles of this interaction, including actin-induced conformational changes that promote actin bundling.

Original languageEnglish (US)
Pages (from-to)697-706
Number of pages10
JournalStructure
Volume22
Issue number5
DOIs
StatePublished - May 6 2014

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Vinculin
Focal Adhesions
Cell Adhesion
Actins
Adherens Junctions
Cytoskeletal Proteins
Traction
Cell Surface Receptors
Molecular Dynamics Simulation
Actin Cytoskeleton
Cell Communication
Mutagenesis
Embryonic Development
Cell Movement
Electron Microscopy
Coloring Agents
Fibroblasts

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Thompson, P. M., Tolbert, C. E., Shen, K., Kota, P., Palmer, S. M., Plevock, K. M., ... Campbell, S. L. (2014). Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties. Structure, 22(5), 697-706. https://doi.org/10.1016/j.str.2014.03.002
Thompson, Peter M. ; Tolbert, Caitlin E. ; Shen, Kai ; Kota, Pradeep ; Palmer, Sean M. ; Plevock, Karen M. ; Orlova, Albina ; Galkin, Vitold E. ; Burridge, Keith ; Egelman, Edward H. ; Dokholyan, Nikolay ; Superfine, Richard ; Campbell, Sharon L. / Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties. In: Structure. 2014 ; Vol. 22, No. 5. pp. 697-706.
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abstract = "Summary Vinculin, a cytoskeletal scaffold protein essential for embryogenesis and cardiovascular function, localizes to focal adhesions and adherens junctions, connecting cell surface receptors to the actin cytoskeleton. While vinculin interacts with many adhesion proteins, its interaction with filamentous actin regulates cell morphology, motility, and mechanotransduction. Disruption of this interaction lowers cell traction forces and enhances actin flow rates. Although a model for the vinculin:actin complex exists, we recently identified actin-binding deficient mutants of vinculin outside sites predicted to bind actin and developed an alternative model to better define this actin-binding surface, using negative-stain electron microscopy (EM), discrete molecular dynamics, and mutagenesis. Actin-binding deficient vinculin variants expressed in vinculin knockout fibroblasts fail to rescue cell-spreading defects and reduce cellular response to external force. These findings highlight the importance of this actin-binding surface and provide the molecular basis for elucidating additional roles of this interaction, including actin-induced conformational changes that promote actin bundling.",
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Thompson, PM, Tolbert, CE, Shen, K, Kota, P, Palmer, SM, Plevock, KM, Orlova, A, Galkin, VE, Burridge, K, Egelman, EH, Dokholyan, N, Superfine, R & Campbell, SL 2014, 'Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties', Structure, vol. 22, no. 5, pp. 697-706. https://doi.org/10.1016/j.str.2014.03.002

Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties. / Thompson, Peter M.; Tolbert, Caitlin E.; Shen, Kai; Kota, Pradeep; Palmer, Sean M.; Plevock, Karen M.; Orlova, Albina; Galkin, Vitold E.; Burridge, Keith; Egelman, Edward H.; Dokholyan, Nikolay; Superfine, Richard; Campbell, Sharon L.

In: Structure, Vol. 22, No. 5, 06.05.2014, p. 697-706.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties

AU - Thompson, Peter M.

AU - Tolbert, Caitlin E.

AU - Shen, Kai

AU - Kota, Pradeep

AU - Palmer, Sean M.

AU - Plevock, Karen M.

AU - Orlova, Albina

AU - Galkin, Vitold E.

AU - Burridge, Keith

AU - Egelman, Edward H.

AU - Dokholyan, Nikolay

AU - Superfine, Richard

AU - Campbell, Sharon L.

PY - 2014/5/6

Y1 - 2014/5/6

N2 - Summary Vinculin, a cytoskeletal scaffold protein essential for embryogenesis and cardiovascular function, localizes to focal adhesions and adherens junctions, connecting cell surface receptors to the actin cytoskeleton. While vinculin interacts with many adhesion proteins, its interaction with filamentous actin regulates cell morphology, motility, and mechanotransduction. Disruption of this interaction lowers cell traction forces and enhances actin flow rates. Although a model for the vinculin:actin complex exists, we recently identified actin-binding deficient mutants of vinculin outside sites predicted to bind actin and developed an alternative model to better define this actin-binding surface, using negative-stain electron microscopy (EM), discrete molecular dynamics, and mutagenesis. Actin-binding deficient vinculin variants expressed in vinculin knockout fibroblasts fail to rescue cell-spreading defects and reduce cellular response to external force. These findings highlight the importance of this actin-binding surface and provide the molecular basis for elucidating additional roles of this interaction, including actin-induced conformational changes that promote actin bundling.

AB - Summary Vinculin, a cytoskeletal scaffold protein essential for embryogenesis and cardiovascular function, localizes to focal adhesions and adherens junctions, connecting cell surface receptors to the actin cytoskeleton. While vinculin interacts with many adhesion proteins, its interaction with filamentous actin regulates cell morphology, motility, and mechanotransduction. Disruption of this interaction lowers cell traction forces and enhances actin flow rates. Although a model for the vinculin:actin complex exists, we recently identified actin-binding deficient mutants of vinculin outside sites predicted to bind actin and developed an alternative model to better define this actin-binding surface, using negative-stain electron microscopy (EM), discrete molecular dynamics, and mutagenesis. Actin-binding deficient vinculin variants expressed in vinculin knockout fibroblasts fail to rescue cell-spreading defects and reduce cellular response to external force. These findings highlight the importance of this actin-binding surface and provide the molecular basis for elucidating additional roles of this interaction, including actin-induced conformational changes that promote actin bundling.

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