Identification of cell surface antigens present on murine hematopoietic stem cells

Barbara Miller, G. Antognetti, T. A. Springer

Research output: Contribution to journalArticle

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Abstract

Nine antigens found on murine bone marrow cells were examined to define their pattern of expression in murine hematopoietic differentiation. Lymphocyte function antigen (LFA-1), heat stable antigen (recognized by M1/69), common leukocyte antigen (CLA, T200, Ly-5) and Lg100a (recognized by 30-C7) were present on early hematopoietic progenitors, BFU-E, CFU-E, CFU-GM, and CFU-M. All antigens found on progenitors were found on some immature precursor cells, myeloblasts, erythroblasts, or monoblasts, but their pattern of expression on identifiable hematopoietic cells varied. Three of these antigens, LFA-1, heat stable antigen recognized by M1/69, and CLA, were expressed on leukocytes of all stages of maturity but were lost from the erythroid lineage during differentiation. MAC-1, Forssman antigen, heat stable antigen (recognized by M1/75), anti-P-95 (recognized by M5/113), and Ia (recognized by M5/114) were found only on differentiated hematopoietic precursors or mature cells. The expression of these antigens was more lineage-specific. MAC-1 and heat stable antigen (recognized by M1/75) were restricted to either mature myeloid or erythroid cells, respectively. The marked differences in distribution of these antigens suggest that they may be useful in negative or positive selection experiments to enrich progenitors, and that some of them may have a functional role in differentiation.

Original languageEnglish (US)
Pages (from-to)3286-3290
Number of pages5
JournalJournal of Immunology
Volume134
Issue number5
StatePublished - Dec 1 1985

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Surface Antigens
Hematopoietic Stem Cells
Antigens
Hot Temperature
Lymphocyte Function-Associated Antigen-1
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Forssman Antigen
Monocyte-Macrophage Precursor Cells
CD45 Antigens
Granulocyte Precursor Cells
Erythroblasts
Erythroid Cells
Myeloid Cells
Bone Marrow Cells
Leukocytes
Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Miller, Barbara ; Antognetti, G. ; Springer, T. A. / Identification of cell surface antigens present on murine hematopoietic stem cells. In: Journal of Immunology. 1985 ; Vol. 134, No. 5. pp. 3286-3290.
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Identification of cell surface antigens present on murine hematopoietic stem cells. / Miller, Barbara; Antognetti, G.; Springer, T. A.

In: Journal of Immunology, Vol. 134, No. 5, 01.12.1985, p. 3286-3290.

Research output: Contribution to journalArticle

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