Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light

Richard J. Wang, B. Tracy Nixon

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Hydrogen peroxide, lethal for human cells, is produced in Dulbecco's modified Eagle's tissue culture medium when exposed to “daylight” fluorescent light. Addition of pure H2O2 and use of the enzyme catalase demonstrate that about 40% of the toxicity in irradiated medium is due to generated peroxide. Riboflavin and tryptophan, or riboflavin and tyrosine, are the components necessary for formation of lethal levels of H2O2 during light exposure.

Original languageEnglish (US)
Pages (from-to)715-722
Number of pages8
JournalIn Vitro
Volume14
Issue number8
DOIs
StatePublished - Jan 1 1978

Fingerprint

fluorescent lighting
Riboflavin
Poisons
riboflavin
Hydrogen Peroxide
tissue culture
hydrogen peroxide
Culture Media
culture media
Light
Eagles
Peroxides
eagles
peroxides
Tryptophan
tryptophan
Catalase
Tyrosine
tyrosine
catalase

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Plant Science

Cite this

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Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light. / Wang, Richard J.; Nixon, B. Tracy.

In: In Vitro, Vol. 14, No. 8, 01.01.1978, p. 715-722.

Research output: Contribution to journalArticle

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