TY - JOUR
T1 - Identification of Listeria monocytogenes from unpasteurized apple juice using rapid test kits
AU - Sado, Patricia N.
AU - Jinneman, Karen C.
AU - Husby, Gary J.
AU - Sorg, Susan M.
AU - Omiecinski, Curtis J.
PY - 1998/9
Y1 - 1998/9
N2 - A microbiological survey of 50 retail juices was conducted in the fall of 1996. These juices were analyzed for Listeria monocytogenes, Escherichia coli O157:H7, Salmonella, coliforms, fecal coliforms, and pH. Two unpasteurized juices were positive for L. monocytogenes: an apple juice and an apple raspberry blend with a pH of 3.78 and 3.75, respectively. Three L. monocytogenes isolates were characterized. The colonies were typical for Listeria sp. on Oxford and lithium chloridephenylethanol-moxalactam agars and were β-hemolytic on sheep blood agar. The isolates required 5 days of incubation at 35°C to produce a positive rhamnose reaction in a phenol red carbohydrate broth. This slow rhamnose utilization resulted in these isolates not being identified using the Micro-ID test strip (Organon Technika). However, the isolates were positive for L. monocytogenes using the API Listeria strip (BioMerieux) and a multiplex polymerase chain reaction for detection of the hemolysis (hyla) and invasion-associated protein (iap) genes.
AB - A microbiological survey of 50 retail juices was conducted in the fall of 1996. These juices were analyzed for Listeria monocytogenes, Escherichia coli O157:H7, Salmonella, coliforms, fecal coliforms, and pH. Two unpasteurized juices were positive for L. monocytogenes: an apple juice and an apple raspberry blend with a pH of 3.78 and 3.75, respectively. Three L. monocytogenes isolates were characterized. The colonies were typical for Listeria sp. on Oxford and lithium chloridephenylethanol-moxalactam agars and were β-hemolytic on sheep blood agar. The isolates required 5 days of incubation at 35°C to produce a positive rhamnose reaction in a phenol red carbohydrate broth. This slow rhamnose utilization resulted in these isolates not being identified using the Micro-ID test strip (Organon Technika). However, the isolates were positive for L. monocytogenes using the API Listeria strip (BioMerieux) and a multiplex polymerase chain reaction for detection of the hemolysis (hyla) and invasion-associated protein (iap) genes.
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U2 - 10.4315/0362-028X-61.9.1199
DO - 10.4315/0362-028X-61.9.1199
M3 - Article
C2 - 9766077
AN - SCOPUS:0032168114
SN - 0362-028X
VL - 61
SP - 1199
EP - 1202
JO - J.MILK.FOOD TECHNOL.
JF - J.MILK.FOOD TECHNOL.
IS - 9
ER -