Identification of [Met5]-enkephalin in developing, adult, and renewing tissues by reversed-phase high performance liquid chromatography and radioimmunoassay

Ian S. Zagon; W. Jeffrey Hurst; Patricia J. McLaughlin

doi:10.1016/S0024-3205(97)00393-7

Identification of [Met⁵]-enkephalin in developing, adult, and renewing tissues by reversed-phase high performance liquid chromatography and radioimmunoassay

Ian S. Zagon, W. Jeffrey Hurst, Patricia J. McLaughlin

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Abstract

Extracts of adult corneal epithelium, and developing and adult cerebellum, aorta, and heart, from rats were evaluated for [Met⁵]- encephalon. Samples were prepared by ultrafiltration and solid phase extraction with a C-18 Sep-pak, separated by reversed phase high-performance liquid chromatography, and analyzed by radioimmunoassay (RIA). This qualitative analysis revealed the presence of [Met⁵]-encephalon in all tissues but the adult cerebellum. These results confirm and extend earlier reports that have used RIA or immunohistochemistry with regard to the presence of this opioid peptide in developing and renewing tissues, and indicate that [Met⁵]-enkephalin is indeed being recognized by immunological assays.

Original language	English (US)
Pages (from-to)	363-370
Number of pages	8
Journal	Life Sciences
Volume	61
Issue number	4
DOIs	https://doi.org/10.1016/S0024-3205(97)00393-7
State	Published - Jun 20 1997

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/S0024-3205(97)00393-7

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title = "Identification of [Met5]-enkephalin in developing, adult, and renewing tissues by reversed-phase high performance liquid chromatography and radioimmunoassay",

abstract = "Extracts of adult corneal epithelium, and developing and adult cerebellum, aorta, and heart, from rats were evaluated for [Met5]- encephalon. Samples were prepared by ultrafiltration and solid phase extraction with a C-18 Sep-pak, separated by reversed phase high-performance liquid chromatography, and analyzed by radioimmunoassay (RIA). This qualitative analysis revealed the presence of [Met5]-encephalon in all tissues but the adult cerebellum. These results confirm and extend earlier reports that have used RIA or immunohistochemistry with regard to the presence of this opioid peptide in developing and renewing tissues, and indicate that [Met5]-enkephalin is indeed being recognized by immunological assays.",

author = "Zagon, {Ian S.} and Hurst, {W. Jeffrey} and McLaughlin, {Patricia J.}",

note = "Funding Information: This work was supported by NIH grants HL-53557, NS-20500, and EY-10300.",

year = "1997",

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T1 - Identification of [Met5]-enkephalin in developing, adult, and renewing tissues by reversed-phase high performance liquid chromatography and radioimmunoassay

AU - Zagon, Ian S.

AU - Hurst, W. Jeffrey

AU - McLaughlin, Patricia J.

N1 - Funding Information: This work was supported by NIH grants HL-53557, NS-20500, and EY-10300.

PY - 1997/6/20

Y1 - 1997/6/20

N2 - Extracts of adult corneal epithelium, and developing and adult cerebellum, aorta, and heart, from rats were evaluated for [Met5]- encephalon. Samples were prepared by ultrafiltration and solid phase extraction with a C-18 Sep-pak, separated by reversed phase high-performance liquid chromatography, and analyzed by radioimmunoassay (RIA). This qualitative analysis revealed the presence of [Met5]-encephalon in all tissues but the adult cerebellum. These results confirm and extend earlier reports that have used RIA or immunohistochemistry with regard to the presence of this opioid peptide in developing and renewing tissues, and indicate that [Met5]-enkephalin is indeed being recognized by immunological assays.

AB - Extracts of adult corneal epithelium, and developing and adult cerebellum, aorta, and heart, from rats were evaluated for [Met5]- encephalon. Samples were prepared by ultrafiltration and solid phase extraction with a C-18 Sep-pak, separated by reversed phase high-performance liquid chromatography, and analyzed by radioimmunoassay (RIA). This qualitative analysis revealed the presence of [Met5]-encephalon in all tissues but the adult cerebellum. These results confirm and extend earlier reports that have used RIA or immunohistochemistry with regard to the presence of this opioid peptide in developing and renewing tissues, and indicate that [Met5]-enkephalin is indeed being recognized by immunological assays.

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Identification of [Met⁵]-enkephalin in developing, adult, and renewing tissues by reversed-phase high performance liquid chromatography and radioimmunoassay

Abstract

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