Identification of microsatellite markers in Plasmodium mexicanum, a lizard malaria parasite that infects nucleated erythrocytes: Primer note

Jos J. Schall, Anne M. Vardo

Research output: Contribution to journalReview article

22 Citations (Scopus)

Abstract

Reptile and bird hosts of malaria parasites (Plasmodium) have nucleated erythrocytes. Infected blood thus contains a mix of abundant host and scant parasite DNA which has prevented identification of Plasmodium microsatellites. We developed a protocol for isolation of microsatellite markers for Plasmodium mexicanum, a parasite of lizards. The ATT repeat was common in the genome of P. mexicanum, but most (87%) of these repeats were exceptionally long (50-206 + repeats). Seven microsatellite markers with polymerase chain reaction primers are described. The protocol should allow discovery of microsatellites of malaria parasites (with AT-rich genomes) infecting bird and reptile hosts.

Original languageEnglish (US)
Pages (from-to)227-229
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number2
DOIs
StatePublished - Mar 1 2007

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Erythroblasts
Lizards
Plasmodium
malaria
lizard
Microsatellite Repeats
Malaria
lizards
parasite
Parasites
erythrocytes
microsatellite repeats
parasites
Reptiles
Birds
reptile
reptiles
genome
Genes
Plasmodium malariae

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "Reptile and bird hosts of malaria parasites (Plasmodium) have nucleated erythrocytes. Infected blood thus contains a mix of abundant host and scant parasite DNA which has prevented identification of Plasmodium microsatellites. We developed a protocol for isolation of microsatellite markers for Plasmodium mexicanum, a parasite of lizards. The ATT repeat was common in the genome of P. mexicanum, but most (87{\%}) of these repeats were exceptionally long (50-206 + repeats). Seven microsatellite markers with polymerase chain reaction primers are described. The protocol should allow discovery of microsatellites of malaria parasites (with AT-rich genomes) infecting bird and reptile hosts.",
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Identification of microsatellite markers in Plasmodium mexicanum, a lizard malaria parasite that infects nucleated erythrocytes : Primer note. / Schall, Jos J.; Vardo, Anne M.

In: Molecular Ecology Notes, Vol. 7, No. 2, 01.03.2007, p. 227-229.

Research output: Contribution to journalReview article

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