Identification of molecular markers linked to the Verticillium wilt resistance gene homologue in potato (Solanum tuberosum L.)

Ivan Simko, Kathleen G. Haynes, Richard W. Jones, Stefano Costanzo, Barbara Jane Christ

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

Verticillium wilt is a vascular disease predominantly caused by the soil-borne fungi Verticillium dahliae and Verticillium albo-atrum. Most of the commercial potato cultivars grown in the USA are susceptible to Verticillium, resulting in significant crop losses. Development of new cultivars with resistance gene(s) against the pathogen can be assisted with molecular marker technology that allows identification and tracking of resistance genes. In tomato, resistance to Verticillium dahliae is conferred by two closely linked genes (Ve1, Ve2) that were mapped to chromosome 9. We have employed primers that amplify the leucine-rich repeat (LRR) domain from tomato Ve1 and Ve2 genes. Verticillium resistance gene homologues have been detected in resistant cv. 'Reddale' when using these primers and genomic DNA as a template. Deduced amino acid sequence shared high identity with Ve1 (87%-90%) and Ve2 (88%-91%) tomato resistance genes. The StVe1 - a potato homologue to the Ve1 gene - mapped to the genomic position corresponding to the tomato Ve1 gene. Microsatellite markers linked to the StVe1 have been used to screen 48 (mostly) tetraploid genotypes of various pedigrees. One of the tested markers showed high linkage with Verticillium resistance (p< 0.001). The correlation is mainly based on the complete absence of resistant genotypes that lack the STM1051 marker (μ190bp size band). The STM1051 marker has a potential use in the detection of genotypes that are susceptible to Verticillium. Our results suggest that there may be a direct evolutionary relationship between Verticillium resistance genes in potato and tomato.

Original languageEnglish (US)
Title of host publicationXXVI International Horticultural Congress
Subtitle of host publicationPotatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton
PublisherInternational Society for Horticultural Science
Pages127-133
Number of pages7
ISBN (Print)9789066053601
DOIs
StatePublished - Jan 1 2003

Publication series

NameActa Horticulturae
Volume619
ISSN (Print)0567-7572

Fingerprint

Verticillium wilt
Solanum tuberosum
potatoes
Verticillium
genetic markers
tomatoes
genes
Verticillium dahliae
genotype
Verticillium albo-atrum
genomics
vascular diseases
crop losses
cultivars
pedigree
tetraploidy
leucine
linkage (genetics)
amino acid sequences
microsatellite repeats

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Simko, I., Haynes, K. G., Jones, R. W., Costanzo, S., & Christ, B. J. (2003). Identification of molecular markers linked to the Verticillium wilt resistance gene homologue in potato (Solanum tuberosum L.). In XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton (pp. 127-133). (Acta Horticulturae; Vol. 619). International Society for Horticultural Science. https://doi.org/10.17660/ActaHortic.2003.619.13
Simko, Ivan ; Haynes, Kathleen G. ; Jones, Richard W. ; Costanzo, Stefano ; Christ, Barbara Jane. / Identification of molecular markers linked to the Verticillium wilt resistance gene homologue in potato (Solanum tuberosum L.). XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. International Society for Horticultural Science, 2003. pp. 127-133 (Acta Horticulturae).
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abstract = "Verticillium wilt is a vascular disease predominantly caused by the soil-borne fungi Verticillium dahliae and Verticillium albo-atrum. Most of the commercial potato cultivars grown in the USA are susceptible to Verticillium, resulting in significant crop losses. Development of new cultivars with resistance gene(s) against the pathogen can be assisted with molecular marker technology that allows identification and tracking of resistance genes. In tomato, resistance to Verticillium dahliae is conferred by two closely linked genes (Ve1, Ve2) that were mapped to chromosome 9. We have employed primers that amplify the leucine-rich repeat (LRR) domain from tomato Ve1 and Ve2 genes. Verticillium resistance gene homologues have been detected in resistant cv. 'Reddale' when using these primers and genomic DNA as a template. Deduced amino acid sequence shared high identity with Ve1 (87{\%}-90{\%}) and Ve2 (88{\%}-91{\%}) tomato resistance genes. The StVe1 - a potato homologue to the Ve1 gene - mapped to the genomic position corresponding to the tomato Ve1 gene. Microsatellite markers linked to the StVe1 have been used to screen 48 (mostly) tetraploid genotypes of various pedigrees. One of the tested markers showed high linkage with Verticillium resistance (p< 0.001). The correlation is mainly based on the complete absence of resistant genotypes that lack the STM1051 marker (μ190bp size band). The STM1051 marker has a potential use in the detection of genotypes that are susceptible to Verticillium. Our results suggest that there may be a direct evolutionary relationship between Verticillium resistance genes in potato and tomato.",
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Simko, I, Haynes, KG, Jones, RW, Costanzo, S & Christ, BJ 2003, Identification of molecular markers linked to the Verticillium wilt resistance gene homologue in potato (Solanum tuberosum L.). in XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. Acta Horticulturae, vol. 619, International Society for Horticultural Science, pp. 127-133. https://doi.org/10.17660/ActaHortic.2003.619.13

Identification of molecular markers linked to the Verticillium wilt resistance gene homologue in potato (Solanum tuberosum L.). / Simko, Ivan; Haynes, Kathleen G.; Jones, Richard W.; Costanzo, Stefano; Christ, Barbara Jane.

XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. International Society for Horticultural Science, 2003. p. 127-133 (Acta Horticulturae; Vol. 619).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AU - Christ, Barbara Jane

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N2 - Verticillium wilt is a vascular disease predominantly caused by the soil-borne fungi Verticillium dahliae and Verticillium albo-atrum. Most of the commercial potato cultivars grown in the USA are susceptible to Verticillium, resulting in significant crop losses. Development of new cultivars with resistance gene(s) against the pathogen can be assisted with molecular marker technology that allows identification and tracking of resistance genes. In tomato, resistance to Verticillium dahliae is conferred by two closely linked genes (Ve1, Ve2) that were mapped to chromosome 9. We have employed primers that amplify the leucine-rich repeat (LRR) domain from tomato Ve1 and Ve2 genes. Verticillium resistance gene homologues have been detected in resistant cv. 'Reddale' when using these primers and genomic DNA as a template. Deduced amino acid sequence shared high identity with Ve1 (87%-90%) and Ve2 (88%-91%) tomato resistance genes. The StVe1 - a potato homologue to the Ve1 gene - mapped to the genomic position corresponding to the tomato Ve1 gene. Microsatellite markers linked to the StVe1 have been used to screen 48 (mostly) tetraploid genotypes of various pedigrees. One of the tested markers showed high linkage with Verticillium resistance (p< 0.001). The correlation is mainly based on the complete absence of resistant genotypes that lack the STM1051 marker (μ190bp size band). The STM1051 marker has a potential use in the detection of genotypes that are susceptible to Verticillium. Our results suggest that there may be a direct evolutionary relationship between Verticillium resistance genes in potato and tomato.

AB - Verticillium wilt is a vascular disease predominantly caused by the soil-borne fungi Verticillium dahliae and Verticillium albo-atrum. Most of the commercial potato cultivars grown in the USA are susceptible to Verticillium, resulting in significant crop losses. Development of new cultivars with resistance gene(s) against the pathogen can be assisted with molecular marker technology that allows identification and tracking of resistance genes. In tomato, resistance to Verticillium dahliae is conferred by two closely linked genes (Ve1, Ve2) that were mapped to chromosome 9. We have employed primers that amplify the leucine-rich repeat (LRR) domain from tomato Ve1 and Ve2 genes. Verticillium resistance gene homologues have been detected in resistant cv. 'Reddale' when using these primers and genomic DNA as a template. Deduced amino acid sequence shared high identity with Ve1 (87%-90%) and Ve2 (88%-91%) tomato resistance genes. The StVe1 - a potato homologue to the Ve1 gene - mapped to the genomic position corresponding to the tomato Ve1 gene. Microsatellite markers linked to the StVe1 have been used to screen 48 (mostly) tetraploid genotypes of various pedigrees. One of the tested markers showed high linkage with Verticillium resistance (p< 0.001). The correlation is mainly based on the complete absence of resistant genotypes that lack the STM1051 marker (μ190bp size band). The STM1051 marker has a potential use in the detection of genotypes that are susceptible to Verticillium. Our results suggest that there may be a direct evolutionary relationship between Verticillium resistance genes in potato and tomato.

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Simko I, Haynes KG, Jones RW, Costanzo S, Christ BJ. Identification of molecular markers linked to the Verticillium wilt resistance gene homologue in potato (Solanum tuberosum L.). In XXVI International Horticultural Congress: Potatoes, Healthy Food for Humanity: International Developments in Breeding, Production, Protection and Utilizaton. International Society for Horticultural Science. 2003. p. 127-133. (Acta Horticulturae). https://doi.org/10.17660/ActaHortic.2003.619.13