Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum

Jean L. Johnson, Neil R. Bastian, Neil L. Schauer, James G. Ferry, K. V. Rajagopalan

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The pterin cofactor in formate dehydrogenase isolated from Methanobacterium formicium is identified as molybdopterin guanine dinucleotide. The pterin, stabilized as the alkylated, dicarboxamidomethyl derivative, is shown to have absorption and chromatographic properties identical to those of the previously characterized authentic compound. Treatment with nucleotide pyrophosphatase produced the expected degradation products GMP and carboxamidomethyl molybdopterin. The molybdopterin guanine dinucleotide released from the enzyme by treatment with 95% dimethyl sulfoxide is shown to be functional in the in vitro reconstitution of the cofactor-deficient nitrate reductase in extracts of the Neurospora crassa nit-1 mutant.

Original languageEnglish (US)
Pages (from-to)213-216
Number of pages4
JournalFEMS Microbiology Letters
Volume77
Issue number2-3
DOIs
StatePublished - Jan 15 1991

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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