@article{9713b7f445b245858148d1c43558dfdf,
title = "Identification of putative binding interface of PI(3,5)P2 lipid on rice black-streaked dwarf virus (RBSDV) P10 protein",
abstract = "Rice black-streaked dwarf virus (RBSDV) is an important reovirus that infects both plants and its transmission vector small brown planthopper, causing severe crop loss. High affinity binding between RBSDV P10 and PI(3,5)P2 lipid layer was measured using biolayer interferometry (BLI). Subcellular co-localization of PI(3,5)P2 and RBSDV P10 was observed on membranous structures in insect cells with stochastic optical reconstruction microscopy (STORM) imaging. Putative interacting sites of PI(3,5)P2 lipid on a computational predicted RBSDV P10 structure were mapped to its “C-domain” (250–470 aa), using HDXMS data. The BLI and STORM results showed binding and co-localization of RBSDV P10, and PI(3,5)P2 on vesicle-like membranous structures were corroborated with the prediction of the binding interface. Understanding the lipid binding sites on viral proteins will lead to developing strategies to block viral-lipid interaction and disrupt viral pathogenesis in insect vectors and to block virus transmission and achieve disease control of crops in the field.",
author = "Haoqiu Liu and Peck, {Xin Yi} and Choong, {Yeu Khai} and Ng, {Woei Shyuan} and Wilfried Engl and Raghuvamsi, {Palur Venkata} and Zhao, {Ziqing Winston} and Anand, {Ganesh S.} and Yijun Zhou and J. Sivaraman and Qiufang Xu and Wong, {Sek Man}",
note = "Funding Information: The authors thank Dr. Markus Wenk from NUS Department of Biochemistry for his suggestions in lipid characterization, Dr. Kini Manjunatha from NUS Department of Biological Sciences for assistance in the biolayer interferometry experiment, Dr. Cho Yeow Koh from NUS Department of Medicine for advice and guidance in setting up the biolayer interferometry, Dr. Junhong Wang and NUS IT{\textquoteright}s Research Computing group for their help in protein structure prediction using AlphaFold2. HDXMS experiments were carried out as a fee for service at the Singapore National Laboratory for Mass Spectrometry (SingMass) funded by National Research Foundation, Singapore . Funding Information: This research was funded by the National Natural Science Foundation of China [ 31872639 ]. Funding Information: This research was funded by the National Natural Science Foundation of China [31872639].The authors thank Dr. Markus Wenk from NUS Department of Biochemistry for his suggestions in lipid characterization, Dr. Kini Manjunatha from NUS Department of Biological Sciences for assistance in the biolayer interferometry experiment, Dr. Cho Yeow Koh from NUS Department of Medicine for advice and guidance in setting up the biolayer interferometry, Dr. Junhong Wang and NUS IT's Research Computing group for their help in protein structure prediction using AlphaFold2. HDXMS experiments were carried out as a fee for service at the Singapore National Laboratory for Mass Spectrometry (SingMass) funded by National Research Foundation, Singapore. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = may,
doi = "10.1016/j.virol.2022.03.010",
language = "English (US)",
volume = "570",
pages = "81--95",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
}