Identification of ring oxidized metabolites of 1-nitropyrene in the feces and urine of germfree f344 rats

Karam El-Bayoumy, Bandaru Reddy, Stephen S. Hecht

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

1-Nitro[U-4,5,9,10-14C]pyrene in trioctanoin was administered by gavage to male germfree F344 rats. Extracts of the 120 h feces and urine were analyzed by h.p.l.c., with or without acetylation. 4,5-Dihydro-4,5-dihydroxy-1-nitropyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6-hydroxypyrene and 1-nitro-8-hydroxypyrene but not 1-aminopyrene, 1-amino-6-hydroxypyrene or 1-amino-8-hydroxypyrene were identified by co-chromatography with standards. These results are in contrast to those obtained in the conventional F344 rat in which 1-aminopyrene, 1-amino-6-hydroxypyrene, and 1-amino-8-Miydroxypyrene were fecal and urinary metabolites, indicating that intestinal microflora are involved in their formation.

Original languageEnglish (US)
Pages (from-to)1371-1373
Number of pages3
JournalCarcinogenesis
Volume5
Issue number10
DOIs
StatePublished - Oct 1 1984

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1-nitropyrene
Inbred F344 Rats
Feces
Urine
Acetylation
Chromatography
1-hydroxypyrene

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

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title = "Identification of ring oxidized metabolites of 1-nitropyrene in the feces and urine of germfree f344 rats",
abstract = "1-Nitro[U-4,5,9,10-14C]pyrene in trioctanoin was administered by gavage to male germfree F344 rats. Extracts of the 120 h feces and urine were analyzed by h.p.l.c., with or without acetylation. 4,5-Dihydro-4,5-dihydroxy-1-nitropyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6-hydroxypyrene and 1-nitro-8-hydroxypyrene but not 1-aminopyrene, 1-amino-6-hydroxypyrene or 1-amino-8-hydroxypyrene were identified by co-chromatography with standards. These results are in contrast to those obtained in the conventional F344 rat in which 1-aminopyrene, 1-amino-6-hydroxypyrene, and 1-amino-8-Miydroxypyrene were fecal and urinary metabolites, indicating that intestinal microflora are involved in their formation.",
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Identification of ring oxidized metabolites of 1-nitropyrene in the feces and urine of germfree f344 rats. / El-Bayoumy, Karam; Reddy, Bandaru; Hecht, Stephen S.

In: Carcinogenesis, Vol. 5, No. 10, 01.10.1984, p. 1371-1373.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of ring oxidized metabolites of 1-nitropyrene in the feces and urine of germfree f344 rats

AU - El-Bayoumy, Karam

AU - Reddy, Bandaru

AU - Hecht, Stephen S.

PY - 1984/10/1

Y1 - 1984/10/1

N2 - 1-Nitro[U-4,5,9,10-14C]pyrene in trioctanoin was administered by gavage to male germfree F344 rats. Extracts of the 120 h feces and urine were analyzed by h.p.l.c., with or without acetylation. 4,5-Dihydro-4,5-dihydroxy-1-nitropyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6-hydroxypyrene and 1-nitro-8-hydroxypyrene but not 1-aminopyrene, 1-amino-6-hydroxypyrene or 1-amino-8-hydroxypyrene were identified by co-chromatography with standards. These results are in contrast to those obtained in the conventional F344 rat in which 1-aminopyrene, 1-amino-6-hydroxypyrene, and 1-amino-8-Miydroxypyrene were fecal and urinary metabolites, indicating that intestinal microflora are involved in their formation.

AB - 1-Nitro[U-4,5,9,10-14C]pyrene in trioctanoin was administered by gavage to male germfree F344 rats. Extracts of the 120 h feces and urine were analyzed by h.p.l.c., with or without acetylation. 4,5-Dihydro-4,5-dihydroxy-1-nitropyrene, 1-nitro-3-hydroxypyrene, 1-nitro-6-hydroxypyrene and 1-nitro-8-hydroxypyrene but not 1-aminopyrene, 1-amino-6-hydroxypyrene or 1-amino-8-hydroxypyrene were identified by co-chromatography with standards. These results are in contrast to those obtained in the conventional F344 rat in which 1-aminopyrene, 1-amino-6-hydroxypyrene, and 1-amino-8-Miydroxypyrene were fecal and urinary metabolites, indicating that intestinal microflora are involved in their formation.

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