Identification of self-incompatibility (S-) locus linked pollen cDNA markers in Petunia inflata

A. G. McCubbin, X. Wang, Teh-hui Kao

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Solanaceous type self-incompatibility (SI) is controlled by a single polymorphic locus, termed the S-locus. The only gene at the S-locus that has been characterized thus far is the S-RNase gene, which controls pistil function, but not pollen function, in SI interactions between pistil and pollen. One approach to identifying additional genes (including the pollen S-gene, which controls pollen function in SI) at the S-locus and to study the structural organization of the S-locus is chromosome walking from the S-RNase gene. However, the presence of highly repetitive sequences in its flanking regions has made this approach difficult so far. Here, we used RNA differential display to identify pollen cDNAs of Petunia inflata, a self-incompatible solanaceous species, which exhibited restriction fragment length polymorphism (RFLP) for at least one of the three S-haplotypes (S1, S2, and S3) examined. We found that the genes corresponding to 10 groups of pollen cDNAs are genetically tightly linked to the S-RNase gene. These cDNA markers will expedite the mapping and cloning of the chromosomal region of the Solanaceae S-locus by providing multiple starting points.

Original languageEnglish (US)
Pages (from-to)619-627
Number of pages9
JournalGenome
Volume43
Issue number4
StatePublished - Sep 18 2000

Fingerprint

Petunia integrifolia
Petunia
Pollen
Complementary DNA
pollen
loci
Genes
genes
pistil
Chromosome Walking
chromosome walking
Solanaceae
repetitive sequences
Nucleic Acid Repetitive Sequences
Restriction Fragment Length Polymorphisms
Haplotypes
Organism Cloning
molecular cloning
haplotypes
restriction fragment length polymorphism

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Genetics

Cite this

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abstract = "Solanaceous type self-incompatibility (SI) is controlled by a single polymorphic locus, termed the S-locus. The only gene at the S-locus that has been characterized thus far is the S-RNase gene, which controls pistil function, but not pollen function, in SI interactions between pistil and pollen. One approach to identifying additional genes (including the pollen S-gene, which controls pollen function in SI) at the S-locus and to study the structural organization of the S-locus is chromosome walking from the S-RNase gene. However, the presence of highly repetitive sequences in its flanking regions has made this approach difficult so far. Here, we used RNA differential display to identify pollen cDNAs of Petunia inflata, a self-incompatible solanaceous species, which exhibited restriction fragment length polymorphism (RFLP) for at least one of the three S-haplotypes (S1, S2, and S3) examined. We found that the genes corresponding to 10 groups of pollen cDNAs are genetically tightly linked to the S-RNase gene. These cDNA markers will expedite the mapping and cloning of the chromosomal region of the Solanaceae S-locus by providing multiple starting points.",
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Identification of self-incompatibility (S-) locus linked pollen cDNA markers in Petunia inflata. / McCubbin, A. G.; Wang, X.; Kao, Teh-hui.

In: Genome, Vol. 43, No. 4, 18.09.2000, p. 619-627.

Research output: Contribution to journalArticle

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AU - Kao, Teh-hui

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