Identification of surface proteins of Spiroplasma citri with protease and antibody probes

Jacqueline Fletcher, John Wills, Sue E. Denman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Spiroplasma citri, a helical, wall-less prokaryote, is an insect-borne phytopathogen. Though proteins having domains on the surface of S. citri cells may be important in pathogenicity or transmissibility, only one surface protein, spiralin (29 KDa), has previously been identified. Intact cells of strain BR3 were treated with chymotrypsin, proteinase K, or trypsin, and the surviving proteins were analyzed by SDS-PAGE. Seven proteins, in addition to spiralin, were degraded, indicative of surface exposure of those polypeptides. Surface immunoprecipitation (SIP) was used to test accessibility of the proteins to anti-S. citri membrane serum, another indication of surface exposure. With unlabeled cells, five such proteins were identified. Four of these have sizes that correspond to those seen with protease treatments. When125I surfacelabeled spiroplasmas were used for SIP, twelve surface proteins were detected, eight of which correspond to bands identified by the other methods. A protein of 89 KDa in strain BR3 was not universally detected in other S. citri strains and spiroplasma species.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalCurrent Microbiology
Volume19
Issue number6
DOIs
StatePublished - Dec 1 1989

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Spiroplasma citri
Membrane Proteins
Peptide Hydrolases
Spiroplasma
Antibodies
Proteins
Immunoprecipitation
Endopeptidase K
Chymotrypsin
Trypsin
Virulence
Insects
Polyacrylamide Gel Electrophoresis
Peptides
Membranes
Serum

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Fletcher, Jacqueline ; Wills, John ; Denman, Sue E. / Identification of surface proteins of Spiroplasma citri with protease and antibody probes. In: Current Microbiology. 1989 ; Vol. 19, No. 6. pp. 383-391.
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Identification of surface proteins of Spiroplasma citri with protease and antibody probes. / Fletcher, Jacqueline; Wills, John; Denman, Sue E.

In: Current Microbiology, Vol. 19, No. 6, 01.12.1989, p. 383-391.

Research output: Contribution to journalArticle

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AB - Spiroplasma citri, a helical, wall-less prokaryote, is an insect-borne phytopathogen. Though proteins having domains on the surface of S. citri cells may be important in pathogenicity or transmissibility, only one surface protein, spiralin (29 KDa), has previously been identified. Intact cells of strain BR3 were treated with chymotrypsin, proteinase K, or trypsin, and the surviving proteins were analyzed by SDS-PAGE. Seven proteins, in addition to spiralin, were degraded, indicative of surface exposure of those polypeptides. Surface immunoprecipitation (SIP) was used to test accessibility of the proteins to anti-S. citri membrane serum, another indication of surface exposure. With unlabeled cells, five such proteins were identified. Four of these have sizes that correspond to those seen with protease treatments. When125I surfacelabeled spiroplasmas were used for SIP, twelve surface proteins were detected, eight of which correspond to bands identified by the other methods. A protein of 89 KDa in strain BR3 was not universally detected in other S. citri strains and spiroplasma species.

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