Identification of terminal flowering1 (TFL1) genes associated with the teak (Tectona grandis) floral development regulation using RNA-seq

D. S. Diningrat, S. M. Widiyanto, A. Pancoro, Iriawati, D. Shim, B. Panchangam, N. Zembower, John Edward Carlson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Teak is woody plants; a member of the Lamiaceae family. Teak is a plant that has a very high quality timber. Teak has constraints due to low reproductive rates and slow growth of the wood after entering the reproductive phase. Teak genetic engineering efforts by delaying flowering time was facing difficulties due to the lack of information about the role of genes regulating flowering identity in teak. Teak has indeterminate inflorescence same as the model plant Arabidopsis. In Arabidopsis, the role of Terminal Flowering 1 (TFL1) gene as a member of the Floral Meristem Identity (FMI) in regulating the vegetative to generative transition is by down regulation, so that, the downstream of the FMI genes up-regulation which resulted in the development towards the formation of flowers. In teak, this mechanism is not well known. The development of NGS technology-transcriptome analysis has allowed us to identify specific interest genes from non-model plant rapidly and cheaply relative. To determine the activity of the interest genes in silico can be undertaken with RNA-seq and QRT-PCR analysis approaches. In this study, it is identified that, TFL1 genes in teak with NGS transcriptome analysis approach that is annotated with S. lycopersicum. The TFL1 genes obtained from EST teak derived from vegetative and generative shoots buds RNA. The TFL1 genes activities on the tissues are done with RNA-seq analysis approach in order to obtain Digitally Gene Expression (DGE) of TFL1. The TFL1 gene activity was then validated in silico by QRT-PCR analysis. The results of the analysis showed that the TFL1-14 gene activity equivalent to the TFL1 gene activity in the model plant.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalResearch Journal of Botany
Volume10
Issue number1
DOIs
StatePublished - Aug 4 2015

Fingerprint

Tectona grandis
RNA
flowering
gene
genes
transcriptomics
meristems
regulation
Arabidopsis
genetic engineering
wood quality
woody plant
Lamiaceae
woody plants
bud
gene expression
analysis
timber
flower
inflorescences

All Science Journal Classification (ASJC) codes

  • Forestry
  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

Cite this

Diningrat, D. S. ; Widiyanto, S. M. ; Pancoro, A. ; Iriawati ; Shim, D. ; Panchangam, B. ; Zembower, N. ; Carlson, John Edward. / Identification of terminal flowering1 (TFL1) genes associated with the teak (Tectona grandis) floral development regulation using RNA-seq. In: Research Journal of Botany. 2015 ; Vol. 10, No. 1. pp. 1-13.
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abstract = "Teak is woody plants; a member of the Lamiaceae family. Teak is a plant that has a very high quality timber. Teak has constraints due to low reproductive rates and slow growth of the wood after entering the reproductive phase. Teak genetic engineering efforts by delaying flowering time was facing difficulties due to the lack of information about the role of genes regulating flowering identity in teak. Teak has indeterminate inflorescence same as the model plant Arabidopsis. In Arabidopsis, the role of Terminal Flowering 1 (TFL1) gene as a member of the Floral Meristem Identity (FMI) in regulating the vegetative to generative transition is by down regulation, so that, the downstream of the FMI genes up-regulation which resulted in the development towards the formation of flowers. In teak, this mechanism is not well known. The development of NGS technology-transcriptome analysis has allowed us to identify specific interest genes from non-model plant rapidly and cheaply relative. To determine the activity of the interest genes in silico can be undertaken with RNA-seq and QRT-PCR analysis approaches. In this study, it is identified that, TFL1 genes in teak with NGS transcriptome analysis approach that is annotated with S. lycopersicum. The TFL1 genes obtained from EST teak derived from vegetative and generative shoots buds RNA. The TFL1 genes activities on the tissues are done with RNA-seq analysis approach in order to obtain Digitally Gene Expression (DGE) of TFL1. The TFL1 gene activity was then validated in silico by QRT-PCR analysis. The results of the analysis showed that the TFL1-14 gene activity equivalent to the TFL1 gene activity in the model plant.",
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Identification of terminal flowering1 (TFL1) genes associated with the teak (Tectona grandis) floral development regulation using RNA-seq. / Diningrat, D. S.; Widiyanto, S. M.; Pancoro, A.; Iriawati; Shim, D.; Panchangam, B.; Zembower, N.; Carlson, John Edward.

In: Research Journal of Botany, Vol. 10, No. 1, 04.08.2015, p. 1-13.

Research output: Contribution to journalArticle

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AU - Carlson, John Edward

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