Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of "candidatus chloracidobacterium thermophilum"

Amaya M.Garcia Costas, Yusuke Tsukatani, W. Irene C. Rijpstra, Stefan Schouten, Paula V. Welander, Roger E. Summons, Donald Ashley Bryant

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

"Candidatus Chloracidobacterium thermophilum" is a recently discovered chlorophototroph from the bacterial phylum Acidobacteria, which synthesizes bacteriochlorophyll (BChl) c and chlorosomes like members of the green sulfur bacteria (GSB) and the green filamentous anoxygenic phototrophs (FAPs). The pigments (BChl c homologs and carotenoids), quinones, lipids, and hopanoids of cells and chlorosomes of this new chlorophototroph were characterized in this study. "Ca. Chloracidobacterium thermophilum" methylates its antenna BChls at the C-8 2 and C-12 1 positions like GSB, but these BChls were esterified with a variety of isoprenoid and straight-chain alkyl alcohols as in FAPs. Unlike the chlorosomes of other green bacteria, "Ca. Chloracidobacterium thermophilum" chlorosomes contained two major xanthophyll carotenoids, echinenone and canthaxanthin. These carotenoids may confer enhanced protection against reactive oxygen species and could represent a specific adaptation to the highly oxic natural environment in which "Ca. Chloracidobacterium thermophilum" occurs. Dihydrogenated menaquinone-8 [menaquinone-8(H 2)], which probably acts as a quencher of energy transfer under oxic conditions, was an abundant component of both cells and chlorosomes of "Ca. Chloracidobacterium thermophilum." The betaine lipid diacylglycerylhydroxymethyl- N, N, N-trimethyl-β;-alanine, esterified with 13-methyl-tetradecanoic (isopentadecanoic) acid, was a prominent polar lipid in the membranes of both "Ca. Chloracidobacterium thermophilum" cells and chlorosomes. This lipid may represent a specific adaptive response to chronic phosphorus limitation in the mats. Finally, three hopanoids, diploptene, bacteriohopanetetrol, and bacteriohopanetetrol cyclitol ether, which may help to stabilize membranes during diel shifts in pH and other physicochemical conditions in the mats, were detected in the membranes of "Ca. Chloracidobacterium thermophilum."

Original languageEnglish (US)
Pages (from-to)1158-1168
Number of pages11
JournalJournal of bacteriology
Volume194
Issue number5
DOIs
StatePublished - Mar 1 2012

Fingerprint

Bacteriochlorophylls
Quinones
Carotenoids
Chlorobi
Lipids
Cyclitols
Acidobacteria
Canthaxanthin
Xanthophylls
Betaine
Membranes
Energy Transfer
Terpenes
Myristic Acid
Cellular Structures
Membrane Lipids
Alanine
Ether
Phosphorus
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Costas, Amaya M.Garcia ; Tsukatani, Yusuke ; Rijpstra, W. Irene C. ; Schouten, Stefan ; Welander, Paula V. ; Summons, Roger E. ; Bryant, Donald Ashley. / Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of "candidatus chloracidobacterium thermophilum". In: Journal of bacteriology. 2012 ; Vol. 194, No. 5. pp. 1158-1168.
@article{9753d75ebcdc4d8f80285c6893ebe79c,
title = "Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of {"}candidatus chloracidobacterium thermophilum{"}",
abstract = "{"}Candidatus Chloracidobacterium thermophilum{"} is a recently discovered chlorophototroph from the bacterial phylum Acidobacteria, which synthesizes bacteriochlorophyll (BChl) c and chlorosomes like members of the green sulfur bacteria (GSB) and the green filamentous anoxygenic phototrophs (FAPs). The pigments (BChl c homologs and carotenoids), quinones, lipids, and hopanoids of cells and chlorosomes of this new chlorophototroph were characterized in this study. {"}Ca. Chloracidobacterium thermophilum{"} methylates its antenna BChls at the C-8 2 and C-12 1 positions like GSB, but these BChls were esterified with a variety of isoprenoid and straight-chain alkyl alcohols as in FAPs. Unlike the chlorosomes of other green bacteria, {"}Ca. Chloracidobacterium thermophilum{"} chlorosomes contained two major xanthophyll carotenoids, echinenone and canthaxanthin. These carotenoids may confer enhanced protection against reactive oxygen species and could represent a specific adaptation to the highly oxic natural environment in which {"}Ca. Chloracidobacterium thermophilum{"} occurs. Dihydrogenated menaquinone-8 [menaquinone-8(H 2)], which probably acts as a quencher of energy transfer under oxic conditions, was an abundant component of both cells and chlorosomes of {"}Ca. Chloracidobacterium thermophilum.{"} The betaine lipid diacylglycerylhydroxymethyl- N, N, N-trimethyl-β;-alanine, esterified with 13-methyl-tetradecanoic (isopentadecanoic) acid, was a prominent polar lipid in the membranes of both {"}Ca. Chloracidobacterium thermophilum{"} cells and chlorosomes. This lipid may represent a specific adaptive response to chronic phosphorus limitation in the mats. Finally, three hopanoids, diploptene, bacteriohopanetetrol, and bacteriohopanetetrol cyclitol ether, which may help to stabilize membranes during diel shifts in pH and other physicochemical conditions in the mats, were detected in the membranes of {"}Ca. Chloracidobacterium thermophilum.{"}",
author = "Costas, {Amaya M.Garcia} and Yusuke Tsukatani and Rijpstra, {W. Irene C.} and Stefan Schouten and Welander, {Paula V.} and Summons, {Roger E.} and Bryant, {Donald Ashley}",
year = "2012",
month = "3",
day = "1",
doi = "10.1128/JB.06421-11",
language = "English (US)",
volume = "194",
pages = "1158--1168",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "5",

}

Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of "candidatus chloracidobacterium thermophilum". / Costas, Amaya M.Garcia; Tsukatani, Yusuke; Rijpstra, W. Irene C.; Schouten, Stefan; Welander, Paula V.; Summons, Roger E.; Bryant, Donald Ashley.

In: Journal of bacteriology, Vol. 194, No. 5, 01.03.2012, p. 1158-1168.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of the bacteriochlorophylls, carotenoids, quinones, lipids, and hopanoids of "candidatus chloracidobacterium thermophilum"

AU - Costas, Amaya M.Garcia

AU - Tsukatani, Yusuke

AU - Rijpstra, W. Irene C.

AU - Schouten, Stefan

AU - Welander, Paula V.

AU - Summons, Roger E.

AU - Bryant, Donald Ashley

PY - 2012/3/1

Y1 - 2012/3/1

N2 - "Candidatus Chloracidobacterium thermophilum" is a recently discovered chlorophototroph from the bacterial phylum Acidobacteria, which synthesizes bacteriochlorophyll (BChl) c and chlorosomes like members of the green sulfur bacteria (GSB) and the green filamentous anoxygenic phototrophs (FAPs). The pigments (BChl c homologs and carotenoids), quinones, lipids, and hopanoids of cells and chlorosomes of this new chlorophototroph were characterized in this study. "Ca. Chloracidobacterium thermophilum" methylates its antenna BChls at the C-8 2 and C-12 1 positions like GSB, but these BChls were esterified with a variety of isoprenoid and straight-chain alkyl alcohols as in FAPs. Unlike the chlorosomes of other green bacteria, "Ca. Chloracidobacterium thermophilum" chlorosomes contained two major xanthophyll carotenoids, echinenone and canthaxanthin. These carotenoids may confer enhanced protection against reactive oxygen species and could represent a specific adaptation to the highly oxic natural environment in which "Ca. Chloracidobacterium thermophilum" occurs. Dihydrogenated menaquinone-8 [menaquinone-8(H 2)], which probably acts as a quencher of energy transfer under oxic conditions, was an abundant component of both cells and chlorosomes of "Ca. Chloracidobacterium thermophilum." The betaine lipid diacylglycerylhydroxymethyl- N, N, N-trimethyl-β;-alanine, esterified with 13-methyl-tetradecanoic (isopentadecanoic) acid, was a prominent polar lipid in the membranes of both "Ca. Chloracidobacterium thermophilum" cells and chlorosomes. This lipid may represent a specific adaptive response to chronic phosphorus limitation in the mats. Finally, three hopanoids, diploptene, bacteriohopanetetrol, and bacteriohopanetetrol cyclitol ether, which may help to stabilize membranes during diel shifts in pH and other physicochemical conditions in the mats, were detected in the membranes of "Ca. Chloracidobacterium thermophilum."

AB - "Candidatus Chloracidobacterium thermophilum" is a recently discovered chlorophototroph from the bacterial phylum Acidobacteria, which synthesizes bacteriochlorophyll (BChl) c and chlorosomes like members of the green sulfur bacteria (GSB) and the green filamentous anoxygenic phototrophs (FAPs). The pigments (BChl c homologs and carotenoids), quinones, lipids, and hopanoids of cells and chlorosomes of this new chlorophototroph were characterized in this study. "Ca. Chloracidobacterium thermophilum" methylates its antenna BChls at the C-8 2 and C-12 1 positions like GSB, but these BChls were esterified with a variety of isoprenoid and straight-chain alkyl alcohols as in FAPs. Unlike the chlorosomes of other green bacteria, "Ca. Chloracidobacterium thermophilum" chlorosomes contained two major xanthophyll carotenoids, echinenone and canthaxanthin. These carotenoids may confer enhanced protection against reactive oxygen species and could represent a specific adaptation to the highly oxic natural environment in which "Ca. Chloracidobacterium thermophilum" occurs. Dihydrogenated menaquinone-8 [menaquinone-8(H 2)], which probably acts as a quencher of energy transfer under oxic conditions, was an abundant component of both cells and chlorosomes of "Ca. Chloracidobacterium thermophilum." The betaine lipid diacylglycerylhydroxymethyl- N, N, N-trimethyl-β;-alanine, esterified with 13-methyl-tetradecanoic (isopentadecanoic) acid, was a prominent polar lipid in the membranes of both "Ca. Chloracidobacterium thermophilum" cells and chlorosomes. This lipid may represent a specific adaptive response to chronic phosphorus limitation in the mats. Finally, three hopanoids, diploptene, bacteriohopanetetrol, and bacteriohopanetetrol cyclitol ether, which may help to stabilize membranes during diel shifts in pH and other physicochemical conditions in the mats, were detected in the membranes of "Ca. Chloracidobacterium thermophilum."

UR - http://www.scopus.com/inward/record.url?scp=84857885821&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857885821&partnerID=8YFLogxK

U2 - 10.1128/JB.06421-11

DO - 10.1128/JB.06421-11

M3 - Article

VL - 194

SP - 1158

EP - 1168

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 5

ER -