Identification of the mammalian Na,K-ATPase β3 subunit

Nasir Malik, Victor Canfield, Marie Claire Beckers, Philippe Gros, Robert Levenson

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

We have isolated and characterized cDNA clones encoding the human and rat Na,K-ATPase β3 subunit isoform. The human cDNA encodes a polypeptide of 279 amino acids that exhibits primary sequence and secondary structure similarities to Na,K-ATPase β subunit isoforms. Sequence comparisons showed that the human β3 subunit closely resembles the β3 subunit of Xenopus laevis (59% amino acid identity) and is less similar to the human Na,K- ATPase β1 and β2 subunits (38% and 48% amino acid identity, respectively). By analyzing the segregation of restriction fragment length polymorphisms among recombinant inbred strains of mice, we localized the β3 subunit gene to murine chromosome 7. Northern blot analysis revealed that the β3 subunit gene encodes two transcripts that are expressed in a variety of rat tissues including testis, brain, kidney, lung, stomach, small intestine, colon, spleen, and liver. Identification of the mammalian β3 subunit suggests an even greater potential for Na,K-ATPase isoenzyme diversity than previously realized.

Original languageEnglish (US)
Pages (from-to)22754-22758
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number37
DOIs
StatePublished - Sep 25 1996

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Adenosine Triphosphatases
Amino Acids
Rats
Protein Isoforms
Complementary DNA
Genes
Inbred Strains Mice
Chromosomes, Human, Pair 7
Xenopus laevis
Chromosomes
Polymorphism
Restriction Fragment Length Polymorphisms
Northern Blotting
Liver
Isoenzymes
Small Intestine
Testis
Brain
Stomach
Colon

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Malik, Nasir ; Canfield, Victor ; Beckers, Marie Claire ; Gros, Philippe ; Levenson, Robert. / Identification of the mammalian Na,K-ATPase β3 subunit. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 37. pp. 22754-22758.
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Identification of the mammalian Na,K-ATPase β3 subunit. / Malik, Nasir; Canfield, Victor; Beckers, Marie Claire; Gros, Philippe; Levenson, Robert.

In: Journal of Biological Chemistry, Vol. 271, No. 37, 25.09.1996, p. 22754-22758.

Research output: Contribution to journalArticle

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