Identification of types II, IX and X collagens at the insertion site of the bovine Achilles tendon

Shoji Fukuta, Masaya Oyama, Karl Kavalkovich, Freddie H. Fu, Christopher Niyibizi

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Achilles tendinous collagen fibrils insert into the calcaneus by first passing through a zone that is defined histologically as fibrocartilaginous. This zone consists of four regions: tendon proper, non-mineralized and mineralized fibrocartilage and bone. The function of this zone has not yet been clearly defined. To gain more insight into the role of this fibrocartilaginous zone, collagens present in the zone of the Achilles tendon-calcaneus interface were isolated and characterized. Types II, IX and X collagens were identified in the pepsin digests of the tissue harvested from the bovine Achilles tendon-calcaneus interface. Western blotting using specific antisera to types II, IX and X collagens confirmed the identity of these collagens. Immunofluorescence localization placed type X collagen predominantly in the mineralized zone of the tendon-calcaneus junction, while type IX collagen was distributed throught the the insertion site. The presence of the cartilage-specific collagens at the Achilles tendon-calcaneus-interface suggests that this zone is cartilaginous in nature. The presence of type X collagen at this junction is not clear, but our present findings go along with the previous report which showed that type X collagen is present in the mineralized zone of the medial collateral ligament femoral insertion site. These data suggest that type X collagen may be a resident of mineralized fibrocartilaginous zones of tendon or ligament-bone junctions and may participate in anchoring ligament or tendon to bone.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalMatrix Biology
Volume17
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

Collagen Type IX
Collagen Type X
Achilles Tendon
Collagen Type II
Calcaneus
Tendons
Collagen
Ligaments
Bone and Bones
Fibrocartilage
Collateral Ligaments
Pepsin A
Thigh
Fluorescent Antibody Technique
Immune Sera
Western Blotting

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Fukuta, Shoji ; Oyama, Masaya ; Kavalkovich, Karl ; Fu, Freddie H. ; Niyibizi, Christopher. / Identification of types II, IX and X collagens at the insertion site of the bovine Achilles tendon. In: Matrix Biology. 1998 ; Vol. 17, No. 1. pp. 65-73.
@article{6f2234b4bc244e318f93c25d4d6b99b1,
title = "Identification of types II, IX and X collagens at the insertion site of the bovine Achilles tendon",
abstract = "Achilles tendinous collagen fibrils insert into the calcaneus by first passing through a zone that is defined histologically as fibrocartilaginous. This zone consists of four regions: tendon proper, non-mineralized and mineralized fibrocartilage and bone. The function of this zone has not yet been clearly defined. To gain more insight into the role of this fibrocartilaginous zone, collagens present in the zone of the Achilles tendon-calcaneus interface were isolated and characterized. Types II, IX and X collagens were identified in the pepsin digests of the tissue harvested from the bovine Achilles tendon-calcaneus interface. Western blotting using specific antisera to types II, IX and X collagens confirmed the identity of these collagens. Immunofluorescence localization placed type X collagen predominantly in the mineralized zone of the tendon-calcaneus junction, while type IX collagen was distributed throught the the insertion site. The presence of the cartilage-specific collagens at the Achilles tendon-calcaneus-interface suggests that this zone is cartilaginous in nature. The presence of type X collagen at this junction is not clear, but our present findings go along with the previous report which showed that type X collagen is present in the mineralized zone of the medial collateral ligament femoral insertion site. These data suggest that type X collagen may be a resident of mineralized fibrocartilaginous zones of tendon or ligament-bone junctions and may participate in anchoring ligament or tendon to bone.",
author = "Shoji Fukuta and Masaya Oyama and Karl Kavalkovich and Fu, {Freddie H.} and Christopher Niyibizi",
year = "1998",
month = "1",
day = "1",
doi = "10.1016/S0945-053X(98)90125-1",
language = "English (US)",
volume = "17",
pages = "65--73",
journal = "Matrix Biology",
issn = "0945-053X",
publisher = "Elsevier",
number = "1",

}

Identification of types II, IX and X collagens at the insertion site of the bovine Achilles tendon. / Fukuta, Shoji; Oyama, Masaya; Kavalkovich, Karl; Fu, Freddie H.; Niyibizi, Christopher.

In: Matrix Biology, Vol. 17, No. 1, 01.01.1998, p. 65-73.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of types II, IX and X collagens at the insertion site of the bovine Achilles tendon

AU - Fukuta, Shoji

AU - Oyama, Masaya

AU - Kavalkovich, Karl

AU - Fu, Freddie H.

AU - Niyibizi, Christopher

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Achilles tendinous collagen fibrils insert into the calcaneus by first passing through a zone that is defined histologically as fibrocartilaginous. This zone consists of four regions: tendon proper, non-mineralized and mineralized fibrocartilage and bone. The function of this zone has not yet been clearly defined. To gain more insight into the role of this fibrocartilaginous zone, collagens present in the zone of the Achilles tendon-calcaneus interface were isolated and characterized. Types II, IX and X collagens were identified in the pepsin digests of the tissue harvested from the bovine Achilles tendon-calcaneus interface. Western blotting using specific antisera to types II, IX and X collagens confirmed the identity of these collagens. Immunofluorescence localization placed type X collagen predominantly in the mineralized zone of the tendon-calcaneus junction, while type IX collagen was distributed throught the the insertion site. The presence of the cartilage-specific collagens at the Achilles tendon-calcaneus-interface suggests that this zone is cartilaginous in nature. The presence of type X collagen at this junction is not clear, but our present findings go along with the previous report which showed that type X collagen is present in the mineralized zone of the medial collateral ligament femoral insertion site. These data suggest that type X collagen may be a resident of mineralized fibrocartilaginous zones of tendon or ligament-bone junctions and may participate in anchoring ligament or tendon to bone.

AB - Achilles tendinous collagen fibrils insert into the calcaneus by first passing through a zone that is defined histologically as fibrocartilaginous. This zone consists of four regions: tendon proper, non-mineralized and mineralized fibrocartilage and bone. The function of this zone has not yet been clearly defined. To gain more insight into the role of this fibrocartilaginous zone, collagens present in the zone of the Achilles tendon-calcaneus interface were isolated and characterized. Types II, IX and X collagens were identified in the pepsin digests of the tissue harvested from the bovine Achilles tendon-calcaneus interface. Western blotting using specific antisera to types II, IX and X collagens confirmed the identity of these collagens. Immunofluorescence localization placed type X collagen predominantly in the mineralized zone of the tendon-calcaneus junction, while type IX collagen was distributed throught the the insertion site. The presence of the cartilage-specific collagens at the Achilles tendon-calcaneus-interface suggests that this zone is cartilaginous in nature. The presence of type X collagen at this junction is not clear, but our present findings go along with the previous report which showed that type X collagen is present in the mineralized zone of the medial collateral ligament femoral insertion site. These data suggest that type X collagen may be a resident of mineralized fibrocartilaginous zones of tendon or ligament-bone junctions and may participate in anchoring ligament or tendon to bone.

UR - http://www.scopus.com/inward/record.url?scp=0031954805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031954805&partnerID=8YFLogxK

U2 - 10.1016/S0945-053X(98)90125-1

DO - 10.1016/S0945-053X(98)90125-1

M3 - Article

C2 - 9628253

AN - SCOPUS:0031954805

VL - 17

SP - 65

EP - 73

JO - Matrix Biology

JF - Matrix Biology

SN - 0945-053X

IS - 1

ER -