IL-2 mRNA levels and degradation rates change with mode of stimulation and phorbol ester treatment of lymphocytes

Olga Bill, Charles G. Garlisi, Deborah S. Grove, Gregory E. Holt, Andrea M. Mastro

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Transient expression of interleukin 2 (IL-2) in activated T lymphocytes may be due to transcriptional and post-transcriptional regulation. As incubation of lymphocytes with the phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) prior to mitogenic stimulation results in decreased levels of IL-2 mRNA, we asked if IL-2 mRNA stability was affected. We found that in TPA-treated cells, IL-2 mRNA was degraded more rapidly than in untreated ones whether the mitogenic stimulus was Concanavalin A (Con A), Con A plus TPA, or TPA plus ionomycin. The degradation was blocked if the TPA pre-incubation included cycloheximide. In contrast, when TPA was included as a co-mitogen, i.e. added at the same time as the mitogen, the IL-2 mRNA levels and stability significantly increased. Compared to the levels found in Con A stimulated cells, TPA plus Con A increased IL-2 mRNA levels by as much as 20-fold and the half-life by 5-fold. TPA plus ionomycin increased the message levels at least 100-fold and half-life by nearly 10-fold. These effects on IL-2 mRNA were not general because IL-2 receptor mRNA stability was not changed even though it also is transiently expressed during the course of lymphocyte activation.

Original languageEnglish (US)
Pages (from-to)102-110
Number of pages9
JournalCytokine
Volume6
Issue number1
DOIs
StatePublished - Jan 1994

Fingerprint

Lymphocytes
RNA Stability
Phorbol Esters
Tetradecanoylphorbol Acetate
Interleukin-2
Acetates
Degradation
Messenger RNA
Concanavalin A
Ionomycin
Mitogens
Half-Life
T-cells
Interleukin-2 Receptors
Cycloheximide
Lymphocyte Activation
Chemical activation
Cells
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Bill, Olga ; Garlisi, Charles G. ; Grove, Deborah S. ; Holt, Gregory E. ; Mastro, Andrea M. / IL-2 mRNA levels and degradation rates change with mode of stimulation and phorbol ester treatment of lymphocytes. In: Cytokine. 1994 ; Vol. 6, No. 1. pp. 102-110.
@article{435c416c9c484f01af0c4c042a388f97,
title = "IL-2 mRNA levels and degradation rates change with mode of stimulation and phorbol ester treatment of lymphocytes",
abstract = "Transient expression of interleukin 2 (IL-2) in activated T lymphocytes may be due to transcriptional and post-transcriptional regulation. As incubation of lymphocytes with the phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) prior to mitogenic stimulation results in decreased levels of IL-2 mRNA, we asked if IL-2 mRNA stability was affected. We found that in TPA-treated cells, IL-2 mRNA was degraded more rapidly than in untreated ones whether the mitogenic stimulus was Concanavalin A (Con A), Con A plus TPA, or TPA plus ionomycin. The degradation was blocked if the TPA pre-incubation included cycloheximide. In contrast, when TPA was included as a co-mitogen, i.e. added at the same time as the mitogen, the IL-2 mRNA levels and stability significantly increased. Compared to the levels found in Con A stimulated cells, TPA plus Con A increased IL-2 mRNA levels by as much as 20-fold and the half-life by 5-fold. TPA plus ionomycin increased the message levels at least 100-fold and half-life by nearly 10-fold. These effects on IL-2 mRNA were not general because IL-2 receptor mRNA stability was not changed even though it also is transiently expressed during the course of lymphocyte activation.",
author = "Olga Bill and Garlisi, {Charles G.} and Grove, {Deborah S.} and Holt, {Gregory E.} and Mastro, {Andrea M.}",
year = "1994",
month = "1",
doi = "10.1016/1043-4666(94)90015-9",
language = "English (US)",
volume = "6",
pages = "102--110",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "1",

}

IL-2 mRNA levels and degradation rates change with mode of stimulation and phorbol ester treatment of lymphocytes. / Bill, Olga; Garlisi, Charles G.; Grove, Deborah S.; Holt, Gregory E.; Mastro, Andrea M.

In: Cytokine, Vol. 6, No. 1, 01.1994, p. 102-110.

Research output: Contribution to journalArticle

TY - JOUR

T1 - IL-2 mRNA levels and degradation rates change with mode of stimulation and phorbol ester treatment of lymphocytes

AU - Bill, Olga

AU - Garlisi, Charles G.

AU - Grove, Deborah S.

AU - Holt, Gregory E.

AU - Mastro, Andrea M.

PY - 1994/1

Y1 - 1994/1

N2 - Transient expression of interleukin 2 (IL-2) in activated T lymphocytes may be due to transcriptional and post-transcriptional regulation. As incubation of lymphocytes with the phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) prior to mitogenic stimulation results in decreased levels of IL-2 mRNA, we asked if IL-2 mRNA stability was affected. We found that in TPA-treated cells, IL-2 mRNA was degraded more rapidly than in untreated ones whether the mitogenic stimulus was Concanavalin A (Con A), Con A plus TPA, or TPA plus ionomycin. The degradation was blocked if the TPA pre-incubation included cycloheximide. In contrast, when TPA was included as a co-mitogen, i.e. added at the same time as the mitogen, the IL-2 mRNA levels and stability significantly increased. Compared to the levels found in Con A stimulated cells, TPA plus Con A increased IL-2 mRNA levels by as much as 20-fold and the half-life by 5-fold. TPA plus ionomycin increased the message levels at least 100-fold and half-life by nearly 10-fold. These effects on IL-2 mRNA were not general because IL-2 receptor mRNA stability was not changed even though it also is transiently expressed during the course of lymphocyte activation.

AB - Transient expression of interleukin 2 (IL-2) in activated T lymphocytes may be due to transcriptional and post-transcriptional regulation. As incubation of lymphocytes with the phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) prior to mitogenic stimulation results in decreased levels of IL-2 mRNA, we asked if IL-2 mRNA stability was affected. We found that in TPA-treated cells, IL-2 mRNA was degraded more rapidly than in untreated ones whether the mitogenic stimulus was Concanavalin A (Con A), Con A plus TPA, or TPA plus ionomycin. The degradation was blocked if the TPA pre-incubation included cycloheximide. In contrast, when TPA was included as a co-mitogen, i.e. added at the same time as the mitogen, the IL-2 mRNA levels and stability significantly increased. Compared to the levels found in Con A stimulated cells, TPA plus Con A increased IL-2 mRNA levels by as much as 20-fold and the half-life by 5-fold. TPA plus ionomycin increased the message levels at least 100-fold and half-life by nearly 10-fold. These effects on IL-2 mRNA were not general because IL-2 receptor mRNA stability was not changed even though it also is transiently expressed during the course of lymphocyte activation.

UR - http://www.scopus.com/inward/record.url?scp=0028292917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028292917&partnerID=8YFLogxK

U2 - 10.1016/1043-4666(94)90015-9

DO - 10.1016/1043-4666(94)90015-9

M3 - Article

C2 - 8003628

AN - SCOPUS:0028292917

VL - 6

SP - 102

EP - 110

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 1

ER -